Linked Life Data

166162

Source:http://linkedlifedata.com/resource/pubmed/id/166162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1975-9-9
pubmed:abstractText
1. Conventional electrophysiological techniques were used to record from isolated rat phrenic nerve-hemidiaphragm preparations. After periods of rest (20 min) or nerve stimulation (7/sec for 20 min) the bathing medium of the preparation was removed and assayed for adenosine triphosphate (ATP) and adenosine diphosphate (ADP) using a sensitive modification of the firefly luciferase method (Silinsky, 1974). 2. In the presence of tubocurarine and normal (2 mM) calcium, fourteen periods of nerve stimulation (eight preparations) caused the appearance of ATP and/or ADP in amounts ranging from 28 to 641 p-mole. Experiments using carbachol (30 muM or 1 mM) suggested that this nucleotide efflux was not produced by a secondary action of released acetylcholine (ACh). 3. Stimulation of isolate phrenic nerve trunks at 7/sec for 20 min did not cause the efflux of ATP or ADP. 4. In solutions of normal osmotic pressure and reduced calcium concentrations (0-1 mM or 'calcium-free'), stimulation failed to release adenine nucleotide from non-contracting preparations. 5. Diaphragms were bathed in normal calcium and indirectly stimulated at 11/sec for 80-90 min in the presence of 5 times 10-minus 5 M hemicholinium-3. After all detectable signs of ACh release were eliminated, nerve stimulation failed to release ATP or ADP. 6. These results in conjunction with experiments on the hydrolysis of exogenous ATP suggest that ATP is released from the motor nerve ending and is subsequently degraded by enzymatic activity. It is also suggested that the released nucleotide may be derived from the cholinergic vesicle.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-13215507, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-13754438, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-13858976, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-13955375, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-14321493, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-16991915, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4152061, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4235865, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4274830, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4297537, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4310002, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4316709, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4323273, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4327744, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4352108, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4354891, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4355233, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4365445, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4374951, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4399351, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4404211, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4451548, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4788046, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-4829966, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-5071932, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-5654681, http://linkedlifedata.com/resource/pubmed/commentcorrection/166162-5921834
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
247
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-62
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
On the association between transmitter secretion and the release of adenine nucleotides from mammalian motor nerve terminals.
pubmed:publicationType
Journal Article, In Vitro