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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-1-28
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pubmed:abstractText |
We have investigated in vivo the coupling between CytR regulation of the deoP2 promoter in Escherichia coli and the DNA-binding specificity of the cAMP-CRP (cAMP receptor protein) complex in order to obtain a more detailed picture of the role played by cAMP-CRP in CytR regulation. By introducing CRP proteins that exhibit an altered DNA binding specificity into a strain containing a mutant deoP2 promoter in which cAMP-CRP activation was decreased and CytR regulation completely abolished, we show that CytR regulation of this promoter can be reestablished by restored the DNA binding of the cAMP-CRP complex. Hence, CytR regulation of deoP2 can be modulated by simply varying DNA binding of cAMP-CRP. These data confirm the crucial role played by the cAMP-CRP activator complex in CytR regulation of the deoP2 promoter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Receptor Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CytR protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Single-Strand Specific DNA and RNA...,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0026-8925
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
231
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
76-80
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1661372-Bacterial Proteins,
pubmed-meshheading:1661372-Base Sequence,
pubmed-meshheading:1661372-Carrier Proteins,
pubmed-meshheading:1661372-Cyclic AMP,
pubmed-meshheading:1661372-Cyclic AMP Receptor Protein,
pubmed-meshheading:1661372-DNA, Bacterial,
pubmed-meshheading:1661372-DNA-Binding Proteins,
pubmed-meshheading:1661372-Escherichia coli,
pubmed-meshheading:1661372-Escherichia coli Proteins,
pubmed-meshheading:1661372-Gene Expression Regulation, Bacterial,
pubmed-meshheading:1661372-Molecular Sequence Data,
pubmed-meshheading:1661372-Plasmids,
pubmed-meshheading:1661372-Promoter Regions, Genetic,
pubmed-meshheading:1661372-Receptors, Cyclic AMP,
pubmed-meshheading:1661372-Recombinant Fusion Proteins,
pubmed-meshheading:1661372-Repressor Proteins,
pubmed-meshheading:1661372-Single-Strand Specific DNA and RNA Endonucleases,
pubmed-meshheading:1661372-beta-Galactosidase
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pubmed:year |
1991
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pubmed:articleTitle |
Restored DNA-binding of the cAMP-CRP activator complex reestablishes negative regulation by the CytR repressor in the deoP2 promoter in Escherichia coli.
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pubmed:affiliation |
Department of Molecular Biology, Odense University, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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