Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-1-29
pubmed:abstractText
Previous studies have suggested that an alteration in the expression of the Na,K-ATPase of muscle may be an important determinant of enhanced insulin sensitivity in chronic renal failure. Therefore, in the present studies we have examined the effect of uremia on the Na,K-ATPase alpha isoforms in skeletal muscle, at the level of mRNA expression and enzymatic activity. The activity of the sodium pump, as measured ouabain-sensitive 86Rb/K uptake in soleus muscle, revealed a reduction in the activity in uremia, related to the increment in plasma creatinine values. The decrement in 86Rb uptake by the rat soleus muscle of experimental animals was associated with changes on Na,K-ATPase gene product. Northern analysis of mRNA revealed isoform-specific regulation of Na,K-ATPase by uremia in skeletal muscle: a decrease of approximately 50% in alpha 1 subunit Na,K-ATPase mRNA, as compared to controls. The decrement in alpha 1 mRNA correlates with the decreased activity of the Na,K-ATPase in uremia, under basal conditions and with the almost complete inhibition of the Na,K-ATPase, of uremic tissue by a concentration of 10(-5) M ouabain. Although the activity of the alpha 2 isoform pump was not modified by uremia, the 3.4-kb message for this enzyme was increased 2.2-fold; this discrepancy is discussed. Altogether these findings demonstrate that the defective extrarenal potassium handling in uremia is at least dependent in the expression of alpha 1 subunit of the Na,K-ATPase.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-1672173, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-1689313, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-1702427, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2167313, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-221488, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2437805, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2447793, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2455720, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2541792, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2682302, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2831227, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2832446, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2837908, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2838907, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2981837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2987824, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-2991273, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-3028470, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-3479802, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-3960016, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-4115981, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-4841371, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-5428349, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-6111930, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-6158890, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-6252786, http://linkedlifedata.com/resource/pubmed/commentcorrection/1661300-7051849
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2137-41
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Effect of chronic renal failure on Na,K-ATPase alpha 1 and alpha 2 mRNA transcription in rat skeletal muscle.
pubmed:affiliation
Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Santiago.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't