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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-12-1
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pubmed:abstractText |
We have previously developed a limbal epithelial culture system using a cell-suspension method on denuded amniotic membrane (AM). However, other workers reported that intact AM is advantageous for limbal epithelial culture in that it preserves stem cell characteristics. In this study, we cultivated human limbal epithelial cell-suspensions on both intact and denuded AM and compared the morphology and adhesion of the limbal epithelial cells on these two substrates.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0721-832X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
245
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-34
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pubmed:dateRevised |
2009-11-3
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pubmed:meshHeading |
pubmed-meshheading:16612639-3T3 Cells,
pubmed-meshheading:16612639-Amnion,
pubmed-meshheading:16612639-Animals,
pubmed-meshheading:16612639-Basement Membrane,
pubmed-meshheading:16612639-Cell Adhesion,
pubmed-meshheading:16612639-Cell Culture Techniques,
pubmed-meshheading:16612639-Cell Differentiation,
pubmed-meshheading:16612639-Coculture Techniques,
pubmed-meshheading:16612639-Epithelial Cells,
pubmed-meshheading:16612639-Epithelium, Corneal,
pubmed-meshheading:16612639-Extracellular Matrix Proteins,
pubmed-meshheading:16612639-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:16612639-Humans,
pubmed-meshheading:16612639-Limbus Corneae,
pubmed-meshheading:16612639-Mice,
pubmed-meshheading:16612639-Microscopy, Electron, Scanning,
pubmed-meshheading:16612639-Microscopy, Electron, Transmission,
pubmed-meshheading:16612639-Stem Cells
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pubmed:year |
2007
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pubmed:articleTitle |
Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells.
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pubmed:affiliation |
Research Center for Regenerative Medicine, Office for Research Initiatives and Development, Doshisha University, Karasuma-Imadegawa, Kamigyoku, 602-8580 Kyoto, Japan. nkoizumi@ophth.kpu-m.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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