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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2006-4-13
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pubmed:abstractText |
Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that infects 40 to 90% of adult human populations. HCMV infections are often asymptomatic in healthy individuals but can cause severe organ and life-threatening disease in immunocompromised patients. The antiviral antibody response to HCMV infection is complex and is known to include virus-neutralizing antibody production against surface glycoproteins encoded by HCMV. We have investigated the human antibody response to a complex of HCMV surface glycoproteins composed of glycoprotein M (gM)/gN, the gene products of the UL100 and UL73 open reading frames. Mouse monoclonal antibodies generated against gM/gN have previously been shown to neutralize HCMV infection of human fibroblasts in vitro. To determine whether human antibodies reactive with the gM/gN complex possess virus-neutralizing properties, we isolated human antibodies reactive with gM/gN from pooled human HCMV hyperimmune globulin by affinity purification using recombinant gM/gN. The affinity-purified human anti-gM/gN antibodies reacted specifically by immunofluorescence with HCMV-infected human fibroblasts and with cells transiently expressing gM/gN, but not with cells transfected with plasmids encoding other immunogenic HCMV proteins. The anti-gM/gN antibodies also reacted specifically only with gM/gN in immunoblot assays using lysates of transfected cells expressing specific HCMV proteins. Last, human anti-gM/gN antibodies efficiently neutralized infectious HCMV in vitro with a capacity comparable to that of human anti-gB antibodies. These data indicated that gM/gN can elicit a virus-neutralizing antibody response in humans infected with HCMV and therefore should be considered a potential candidate for inclusion in prophylactic CMV vaccines.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-10379942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-10466818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-10623760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-10888614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-11090188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-11213930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-11773418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-11861861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-11922941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-12364375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-12604817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-12771417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-1309850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-15307033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-15452216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-15681419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-1658156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-1663509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-1702157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-2467992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-2536837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-2542350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-2547996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-2833009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-3002068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-3037013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-3670292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-6328055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-6330979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-7679747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-7681609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-8381465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-8389812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-8393078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-8757997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9268149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9292000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9420258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9525625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9525659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9621013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16611919-9882288
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4591-600
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16611919-Absorption,
pubmed-meshheading:16611919-Animals,
pubmed-meshheading:16611919-Antibodies, Viral,
pubmed-meshheading:16611919-Cell Line,
pubmed-meshheading:16611919-Cercopithecus aethiops,
pubmed-meshheading:16611919-Chromatography, Affinity,
pubmed-meshheading:16611919-Cytomegalovirus,
pubmed-meshheading:16611919-Cytomegalovirus Infections,
pubmed-meshheading:16611919-Humans,
pubmed-meshheading:16611919-Immunoglobulin G,
pubmed-meshheading:16611919-Viral Envelope Proteins,
pubmed-meshheading:16611919-Virion
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pubmed:year |
2006
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pubmed:articleTitle |
Human cytomegalovirus infection elicits a glycoprotein M (gM)/gN-specific virus-neutralizing antibody response.
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pubmed:affiliation |
Department of Pediatrics, University of Alabama at Birmingham, 1600 6th Avenue South, CHB107, Birmingham, Alabama 35233, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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