Linked Life Data

16611734

Source:http://linkedlifedata.com/resource/pubmed/id/16611734

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-8-9
pubmed:abstractText
Myostatin (GDF-8) is known to negatively regulate skeletal muscle mass in myogenesis, but few studies have been conducted on the function of endogenous GDF-8 in primary myoblasts. The present study was performed to assess the function of GDF-8 by RNA interference using primary culture of chicken embryonic myoblasts in which myoblasts were differentiated into myotubes. An active form of small interfering RNA (siRNA-1) targeting GDF-8 mRNA was introduced into myoblasts, and an inactive form of siRNA (siRNA-2) was used as a negative control. GDF-8 transcript level was significantly reduced 24 h after the introduction of siRNA-1 to 25% of the control, whereas a 52-kDa GDF-8 precursor was reduced to 45% of the control at 48 h. However, siRNA-2 did not decrease GDF-8 transcript level. When GDF-8-mediated promoter activity was measured chronologically by means of a pGL(CAGA)(10)-constructed luciferase reporter assay, a concomitant change in activity was initiated after 24 h. The activity rapidly decreased 30 h after siRNA-1 introduction, whereas high activity was maintained at 30-42 h in the control and siRNA-2-treated myoblasts. Myogenic factors such as MyoD and p21, but not myogenin, were altered after 72 h. Cell fusion of the multinucleated myotubes was delayed by the siRNA-1 introduction, and myotubes with aggregated nuclei were shorter and wider. These results strongly suggest that deficiency of GDF-8 delays cell differentiation and causes great alterations in the cellular morphology of chicken embryonic myotubes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
291
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C538-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16611734-Animals, pubmed-meshheading:16611734-Cell Culture Techniques, pubmed-meshheading:16611734-Cell Differentiation, pubmed-meshheading:16611734-Cell Shape, pubmed-meshheading:16611734-Chick Embryo, pubmed-meshheading:16611734-Culture Media, Serum-Free, pubmed-meshheading:16611734-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:16611734-Muscle Fibers, Skeletal, pubmed-meshheading:16611734-MyoD Protein, pubmed-meshheading:16611734-Myoblasts, pubmed-meshheading:16611734-Myogenin, pubmed-meshheading:16611734-Myostatin, pubmed-meshheading:16611734-RNA, Small Interfering, pubmed-meshheading:16611734-RNA Interference, pubmed-meshheading:16611734-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16611734-Transfection, pubmed-meshheading:16611734-Transforming Growth Factor beta
pubmed:year
2006
pubmed:articleTitle
Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA.
pubmed:affiliation
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Graduate School Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't