Linked Life Data

16611231

Source:http://linkedlifedata.com/resource/pubmed/id/16611231

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-4-13
pubmed:abstractText
We have previously shown that Actinobacillus actinomycetemcomitans cytolethal-distending toxin (Cdt) is a potent immunosuppressive agent that induces G2/M arrest in human lymphocytes. In this study, we explored the possibility that Cdt-mediated immunotoxicity involves lipid membrane microdomains. We first determined that following treatment of Jurkat cells with Cdt holotoxin all three Cdt subunits localize to these microdomains. Laser confocal microscopy was employed to colocalize the subunits with GM1-enriched membrane regions which are characteristic of membrane rafts. Western blot analysis of isolated lipid rafts also demonstrated the presence of Cdt peptides. Cholesterol depletion, using methyl beta-cyclodextrin, protected cells from the ability of the Cdt holotoxin to induce G2 arrest. Moreover, cholesterol depletion reduced the ability of the toxin to associate with Jurkat cells. Thus, lipid raft integrity is vital to the action of Cdt on host cells. The implications of our observations with respect to Cdt mode of action are discussed.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/DE06014, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-06, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-07, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-08, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-09, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-10A2, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-11, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-12, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-13A2, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-14, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-15, http://linkedlifedata.com/resource/pubmed/grant/R01 EY010420-16, http://linkedlifedata.com/resource/pubmed/grant/R21 EY018705-01A1, http://linkedlifedata.com/resource/pubmed/grant/R21 EY018705-02, http://linkedlifedata.com/resource/pubmed/grant/R29 EY010420-03, http://linkedlifedata.com/resource/pubmed/grant/S10 RR026365-01
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1462-5814
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
823-36
pubmed:dateRevised
2011-10-20
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin.
pubmed:affiliation
Department of Biochemistry, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA. battagli@biochem.dental.upenn.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural