Linked Life Data

16604286

Source:http://linkedlifedata.com/resource/pubmed/id/16604286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-5-12
pubmed:abstractText
The fabrication of surfaces that stimulate increased adhesion, migration, and differentiated function of osteoblasts has been viewed as being desirable for many orthopedic applications. Previous studies have shown that microfabricated pits and grooves alter adhesion, spreading, matrix secretion, and production of mineral by rat calvarial osteoblasts (RCOs). The mechanisms underlying these effects are unknown, although microenvironment and cell alignment are considered to play a role. The aim of this work was to investigate the behavior of RCOs on microfabricated discontinuous-edge surfaces (DESs), which could provide an alternative means to control both the microenvironment and cellular alignment. Two types of discontinuous-type structures were employed, gap-cornered boxes and micron scale pillars. DES gap-cornered boxes and the pillars influenced the arrangement of F-actin, microtubules, and vinculin. Osteoblasts were guided in their direction of migration on both types of substrata. Both box DESs and pillars altered the staining intensity and localization pattern of phosphotyrosine and src-activated FAK localization. Cell multilayering, matrix deposition, and mineralization were enhanced on both discontinuous topographies when compared with smooth controls. This study shows that DESs alter adhesion, migration, and proliferative responses from osteoblasts at early time points (<1 week) and promote multilayering, matrix deposition, and mineral deposition at later times (2-6 weeks). Such topographical patterns could potentially be employed as effective surface features on bone-contacting implants or in membrane-based periodontal applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0171-967X
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
314-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16604286-Actins, pubmed-meshheading:16604286-Alkaline Phosphatase, pubmed-meshheading:16604286-Animals, pubmed-meshheading:16604286-Bone Matrix, pubmed-meshheading:16604286-Bone Regeneration, pubmed-meshheading:16604286-Calcification, Physiologic, pubmed-meshheading:16604286-Cell Adhesion, pubmed-meshheading:16604286-Cell Movement, pubmed-meshheading:16604286-Cell Proliferation, pubmed-meshheading:16604286-Cells, Cultured, pubmed-meshheading:16604286-Cytoskeleton, pubmed-meshheading:16604286-Epoxy Resins, pubmed-meshheading:16604286-Focal Adhesion Protein-Tyrosine Kinases, pubmed-meshheading:16604286-Microscopy, Electron, Scanning, pubmed-meshheading:16604286-Microscopy, Electron, Transmission, pubmed-meshheading:16604286-Microscopy, Video, pubmed-meshheading:16604286-Microtubules, pubmed-meshheading:16604286-Osteoblasts, pubmed-meshheading:16604286-Osteogenesis, pubmed-meshheading:16604286-Phosphorylation, pubmed-meshheading:16604286-Phosphotyrosine, pubmed-meshheading:16604286-Rats, pubmed-meshheading:16604286-Skull, pubmed-meshheading:16604286-Vinculin
pubmed:year
2006
pubmed:articleTitle
Microfabricated discontinuous-edge surface topographies influence osteoblast adhesion, migration, cytoskeletal organization, and proliferation and enhance matrix and mineral deposition in vitro.
pubmed:affiliation
Department of Oral, Biological, and Medical Sciences, University of British Columbia, 2199 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't