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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-4-10
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pubmed:abstractText |
To investigate the changes in drug sensitivity of Bcl-2 siRNA transfected HepG2 cells. Bcl-2 siRNA and negative siRNA expression vector were constructed and stably transfected into HepG2 cells. RT-PCR and Immunofluorescence were used to detect the target gene expression. Western Blotting was used to detect Bcl-2, Bax and caspase-3 protein expressiom. Drug sensitivity of the cells to 5-fluorouracil (5-FU) and 10-hydroxycamptothecin (HCPT) were analyzed with MTT and flow cytometry. Results were following: (1) the mRNA and protein expression level of Bcl-2 in Bcl-2 siRNA stable transfectants were reduced compared with negative siRNA transfected or untreated cells. Accordingly, Bax protein expression had no change and caspase-3 protein expression showed significantly be up regulated; (2) MTT results showed that Bcl-2 siRNA transfectants had higher cell inhibitory rates after treated with 5-FU or HCPT; (3) flow cytometry results demonstrated that sub G1 population increased in Bcl-2 siRNA transfected cells compared with negative siRNA or untreated cells. After addition 5-FU (1300 mg/l) and HCPT (0.72 mg/l), Bcl-2 siRNA cells showed higher sub G1 population than negative siRNA or untreated cells. siRNA targeting Bcl-2 gene can specifically down-regulate Bcl-2 expression, increased Bax/Bcl-2 ratio expression and caspase-3 activity in HepG2 cells, which lead to increase cells spontaneous apoptosis and sensitize cells to 5-FU or HCPT. Bcl-2 siRNA may be a potential therapy agent against human hepatoblastoma.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/10-hydroxycamptothecin,
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Camptothecin,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1061-186X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16603448-Antimetabolites, Antineoplastic,
pubmed-meshheading:16603448-Apoptosis,
pubmed-meshheading:16603448-Blotting, Western,
pubmed-meshheading:16603448-Camptothecin,
pubmed-meshheading:16603448-Caspase 3,
pubmed-meshheading:16603448-Caspases,
pubmed-meshheading:16603448-Cell Line, Tumor,
pubmed-meshheading:16603448-Drug Delivery Systems,
pubmed-meshheading:16603448-Drug Synergism,
pubmed-meshheading:16603448-Flow Cytometry,
pubmed-meshheading:16603448-Fluorouracil,
pubmed-meshheading:16603448-Genes, bcl-2,
pubmed-meshheading:16603448-Humans,
pubmed-meshheading:16603448-Liver Neoplasms,
pubmed-meshheading:16603448-RNA, Small Interfering,
pubmed-meshheading:16603448-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16603448-Tetrazolium Salts,
pubmed-meshheading:16603448-Thiazoles,
pubmed-meshheading:16603448-bcl-2-Associated X Protein
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pubmed:year |
2006
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pubmed:articleTitle |
Bcl-2 siRNA induced apoptosis and increased sensitivity to 5-fluorouracil and HCPT in HepG2 cells.
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pubmed:affiliation |
Institute of Pharmarcy and Pharmacology, Nanhua University, Hengyang, 421001, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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