Linked Life Data

16601955

Source:http://linkedlifedata.com/resource/pubmed/id/16601955

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-4-21
pubmed:abstractText
The development of novel solid phases enabled us to create affinity resins that could be used to isolate the whole complex of target proteins responsible for the immunosuppressive effects of FK506 from rat brain lysate, whereas the affinity resins from commercially available matrices could not achieve this isolation. The results illustrate the enhanced effectiveness of the affinity resin made from this novel material at identifying the target protein of the bioactive compound compared to resins made from the well-known materials Affigel or Toyopearl. This effectiveness arises because the novel material is hydrophilic enough to reduce nonspecific binding proteins and because it has a higher density of ligands that capture the nonubiquitous target protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1618-2642
pubmed:author
pubmed:issnType
Print
pubmed:volume
385
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
122-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Isolating the whole complex of target proteins of FK506 using affinity resins from novel solid phases.
pubmed:affiliation
Chemistry Department, Reverse Proteomics Research Institute Co., Ltd., 2-6-7 Kazusa-Kamatari, Chiba, 292-0818, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't