Source:http://linkedlifedata.com/resource/pubmed/id/16600246
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2006-5-29
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| pubmed:databankReference | |
| pubmed:abstractText |
This study examines the biology of NLP-12 neuropeptides in Caenorhabditis elegans, and in the parasitic nematodes Ascaris suum and Trichostrongylus colubriformis. DYRPLQFamide (1 nM-10 microM; n > or =6) produced contraction of innervated dorsal and ventral Ascaris body wall muscle preparations (10 microM, 6.8+/-1.9 g; 1 microM, 4.6+/-1.8 g; 0.1 microM, 4.1+/-2.0 g; 10 nM, 3.8+/-2.0 g; n > or =6), and also caused a qualitatively similar, but quantitatively lower contractile response (10 microM, 4.0+/-1.5 g, n=6) on denervated muscle strips. Ovijector muscle displayed no measurable response (10 microM, n=5). nlp-12 cDNAs were characterised from A. suum (As-nlp-12) and T. colubriformis (Tc-nlp-12), both of which show sequence similarity to C. elegans nlp-12, in that they encode multiple copies of -LQFamide peptides. In C. elegans, reverse transcriptase (RT)-PCR analysis showed that nlp-12 was transcribed throughout the life cycle, suggesting that DYRPLQFamide plays a constitutive role in the nervous system of this nematode. Transcription was also identified in both L3 and adult stages of T. colubriformis, in which Tc-nlp-12 is expressed in a single tail neurone. Conversely, As-nlp-12 is expressed in both head and tail tissue of adult female A. suum, suggesting species-specific differences in the transcription pattern of this gene.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0020-7519
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
31
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| pubmed:volume |
36
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
633-40
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| pubmed:meshHeading |
pubmed-meshheading:16600246-Amino Acid Sequence,
pubmed-meshheading:16600246-Animals,
pubmed-meshheading:16600246-Ascaris suum,
pubmed-meshheading:16600246-Caenorhabditis elegans,
pubmed-meshheading:16600246-Caenorhabditis elegans Proteins,
pubmed-meshheading:16600246-DNA, Complementary,
pubmed-meshheading:16600246-DNA, Helminth,
pubmed-meshheading:16600246-Dose-Response Relationship, Drug,
pubmed-meshheading:16600246-Female,
pubmed-meshheading:16600246-Gene Expression,
pubmed-meshheading:16600246-Helminth Proteins,
pubmed-meshheading:16600246-Male,
pubmed-meshheading:16600246-Molecular Sequence Data,
pubmed-meshheading:16600246-Muscle Contraction,
pubmed-meshheading:16600246-Nematoda,
pubmed-meshheading:16600246-Neuropeptides,
pubmed-meshheading:16600246-Tissue Culture Techniques,
pubmed-meshheading:16600246-Transcription, Genetic
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| pubmed:year |
2006
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| pubmed:articleTitle |
Gene expression and pharmacology of nematode NLP-12 neuropeptides.
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| pubmed:affiliation |
Parasitology Research Group, School of Biology and Biochemistry, Medical Biology Centre, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK. paul.mcveigh@qub.ac.uk
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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