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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-1-2
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pubmed:databankReference |
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pubmed:abstractText |
Multiple catalytic subunits of the Ca2+ and calmodulin (CaM)-dependent protein phosphatase (PrP) ("calcineurin" or PrP-2B) are derived from at least two structural genes, type 1 ("calcineurin A alpha") and type 2 ("calcineurin A beta "), each of which can produce alternatively spliced transcripts. To examine the possible linkage of these genes, we analyzed genomic DNA from human/hamster hybrid cell lines using probes of 122 base pairs that were designed to bind selectively to exon 3 of the open reading frame. In this region, the nucleotide sequence of the type 2 murine cDNA that we cloned was greater than 99% identical to the type 2 human cDNA but only 78% identical to the type 1 human cDNA. Hybridization to Southern blots containing DNA from all human chromosomes showed that gene 1 was found on chromosome 4, whereas gene 2 segregated to chromosome 10. These data suggest that expression of the two calcineurin genes is not physically linked.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
252-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1659808-Amino Acid Sequence,
pubmed-meshheading:1659808-Animals,
pubmed-meshheading:1659808-Base Sequence,
pubmed-meshheading:1659808-Blotting, Southern,
pubmed-meshheading:1659808-Calcineurin,
pubmed-meshheading:1659808-Calmodulin-Binding Proteins,
pubmed-meshheading:1659808-Chromosomes, Human, Pair 10,
pubmed-meshheading:1659808-Chromosomes, Human, Pair 2,
pubmed-meshheading:1659808-Cloning, Molecular,
pubmed-meshheading:1659808-Cricetinae,
pubmed-meshheading:1659808-DNA,
pubmed-meshheading:1659808-Genes,
pubmed-meshheading:1659808-Humans,
pubmed-meshheading:1659808-Hybrid Cells,
pubmed-meshheading:1659808-Macromolecular Substances,
pubmed-meshheading:1659808-Mice,
pubmed-meshheading:1659808-Molecular Sequence Data,
pubmed-meshheading:1659808-Nucleic Acid Hybridization,
pubmed-meshheading:1659808-Phosphoprotein Phosphatases,
pubmed-meshheading:1659808-Restriction Mapping,
pubmed-meshheading:1659808-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1659808-Thymus Gland
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pubmed:year |
1991
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pubmed:articleTitle |
Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit.
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pubmed:affiliation |
Section on Immunology, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852.
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pubmed:publicationType |
Journal Article,
Comparative Study
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