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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-4-6
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pubmed:abstractText |
Isothermal nucleic acid sequence-based amplification (NASBA) was applied to the detection of Chlamydophila pneumoniae 16S rRNA by using the NucliSens basic kit (bioMérieux, Boxtel, The Netherlands). The assay was originally developed as a conventional NASBA assay with electrochemiluminescence detection and was subsequently adapted to a real-time NASBA format by using a molecular beacon. C. pneumoniae RNA prepared from a plasmid construct was used to assess the analytical sensitivity of the assay. The sensitivity of the NASBA assay was 10 molecules of in vitro wild-type C. pneumoniae RNA and 0.1 inclusion-forming unit (IFU) of C. pneumoniae. In spiked respiratory specimens, the sensitivity of the C. pneumoniae NASBA assay varied between 0.1 and 1 IFU/100 mul sample, depending on the type of specimen. Finally, conventional and real-time NASBA were applied to respiratory specimens previously tested by PCR. A 100% concordance between the test results was obtained.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-10390424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-10974144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-11283059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-11744436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-11923354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-12732431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-12958290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-1597013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-1691208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-2181028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-7531141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-7594672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-7622912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-8195733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-8248741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-8307044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-8711652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-8783693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-9180199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-9300385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-9547273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-9571739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16597845-9727200
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1241-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16597845-Chlamydophila pneumoniae,
pubmed-meshheading:16597845-DNA, Bacterial,
pubmed-meshheading:16597845-Humans,
pubmed-meshheading:16597845-Polymerase Chain Reaction,
pubmed-meshheading:16597845-RNA, Ribosomal, 16S,
pubmed-meshheading:16597845-Respiratory System,
pubmed-meshheading:16597845-Self-Sustained Sequence Replication,
pubmed-meshheading:16597845-Sensitivity and Specificity
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pubmed:year |
2006
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pubmed:articleTitle |
Development of conventional and real-time nucleic acid sequence-based amplification assays for detection of Chlamydophila pneumoniae in respiratory specimens.
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pubmed:affiliation |
Department of Medical Microbiology, University of Antwerp, Universiteitsplein 1 S3, B-2610 Wilrijk, Belgium. katherine.loens@ua.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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