Source:http://linkedlifedata.com/resource/pubmed/id/16596255
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2006-4-5
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pubmed:abstractText |
Early detection is the hallmark of successful cancer treatment. Evidence is accumulating that primary cancers begin shedding neoplastic cells in the circulation at an early stage. To date, a high-sensitivity and high-throughput method for the detection of circulating tumor cells (CTCs) is deficient. In this study, we have developed a high-sensitivity colorimetric membrane-array method to detect CTCs in the peripheral blood of colorectal cancer (CRC) patients as a potential diagnostic tool. Previously, we identified a set of 18 oligonucleotide clones, significantly overexpressed in CRC, which were synthesized and applied to a nylon membrane. Digoxigenin (DIG)-labeled cDNA were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) from the peripheral blood of 88 Taiwanese CRC patients and 50 healthy subjects, and were then hybridized to the membrane-array. Hybridization signals were detected by color development. Meanwhile, blood samples were analyzed by real-time quantitative PCR (Q-PCR). Subsequently, both methods were compared regarding their correlation, sensitivity and specificity in the detection of CTCs by statistics. The results of membrane-arrays were demonstrated to be closely related to that of Q-PCR (P<0.001). The sensitivity and specificity of membrane-arrays for the detection of CTCs were 94.3% (95% CI, 86.4-102.2%) and 94% (95% CI, 85.9-102.1%), respectively. Moreover, the accuracy of membrane-arrays is higher than that of any one gene by Q-PCR. The detection rate of membrane-arrays was significantly associated with the depth of tumor invasion (P=0.002), the presence of lymph node metastasis (P=0.016), and TNM stage (P=0.005). The preliminary results indicated that the accuracy of membrane-arrays was sufficient to distinguish Taiwanese CRC patients from normal individuals with the advantages of time-saving, cost-effectiveness and high-throughput. Thus, the constructed colorimetric membrane-array could be a promising approach for the future detection of CTCs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1107-3756
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
737-47
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16596255-Adult,
pubmed-meshheading:16596255-Aged,
pubmed-meshheading:16596255-Aged, 80 and over,
pubmed-meshheading:16596255-Colorectal Neoplasms,
pubmed-meshheading:16596255-Digoxigenin,
pubmed-meshheading:16596255-Female,
pubmed-meshheading:16596255-Gene Expression Profiling,
pubmed-meshheading:16596255-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:16596255-Humans,
pubmed-meshheading:16596255-Linear Models,
pubmed-meshheading:16596255-Male,
pubmed-meshheading:16596255-Middle Aged,
pubmed-meshheading:16596255-Neoplastic Cells, Circulating,
pubmed-meshheading:16596255-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16596255-Reproducibility of Results,
pubmed-meshheading:16596255-Sensitivity and Specificity,
pubmed-meshheading:16596255-Taiwan
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pubmed:year |
2006
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pubmed:articleTitle |
Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer.
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pubmed:affiliation |
Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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