Source:http://linkedlifedata.com/resource/pubmed/id/16596173
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2006-4-5
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pubmed:abstractText |
E-cadherin and DAP kinase have been implicated as 'invasion suppressor' genes in human cancer. The aim of this study was to analyze the methylation status of E-cadherin and DAP kinase and the expression of the protein in the metastatic lesions and to compare it with the expression in the primary tumor. Methylation-specific PCR of the DAP kinase and E-cadherin promoter was performed in 28 primary adenocarcinomas of the pancreas and in 13 corresponding regional lymph node metastases. The presence of E-cadherin and DAP kinase protein was assessed by immunohistochemistry. Metastatic lymph nodes showed a significant different expression profile from the primary tumor. E-cadherin methylation was observed in 8/28 (29%) and loss of protein expression was observed in 16/28 (57%) of pancreatic carcinomas. E-cadherin methylation was observed in 7/13 (54%) and loss of protein expression was observed in 11/13 (85%) lymph node metastases (p=0.047). DAP kinase methylation occurred in 11/28 (39%) pancreatic carcinomas and loss of protein expression was observed in 13/28 (46%). DAP kinase was methylated in 6/13 (46%) lymph node meta-stases and loss of protein expression was observed in 10/13 (77%) (p=0.039). Comparing primary tumor and corresponding lymph node metastases in 13 cases, the status of E-cadherin methylation was discordant in 2 cases. The protein expression pattern of E-cadherin and DAP kinase was discordant in 4 and 3 cases respectively. Unmethylated tumor samples did not express E-cadherin in 12 and DAP kinase protein in 6 cases. Our results demonstrate that reduction of E-cadherin and DAP kinase expression is more frequent in lymph node metastases than in the primary tumor and methylation of the promoter region contributes to this reduction; however, an alternative mechanism of inactivation seems to exist.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/death-associated protein kinase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1021-335X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1125-31
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16596173-Adenocarcinoma,
pubmed-meshheading:16596173-Aged,
pubmed-meshheading:16596173-Apoptosis Regulatory Proteins,
pubmed-meshheading:16596173-Cadherins,
pubmed-meshheading:16596173-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:16596173-Carcinoma, Pancreatic Ductal,
pubmed-meshheading:16596173-DNA Methylation,
pubmed-meshheading:16596173-Female,
pubmed-meshheading:16596173-Gene Expression Profiling,
pubmed-meshheading:16596173-Humans,
pubmed-meshheading:16596173-Lymphatic Metastasis,
pubmed-meshheading:16596173-Male,
pubmed-meshheading:16596173-Middle Aged,
pubmed-meshheading:16596173-Neoplasm Staging,
pubmed-meshheading:16596173-Pancreatic Neoplasms,
pubmed-meshheading:16596173-Promoter Regions, Genetic
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pubmed:year |
2006
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pubmed:articleTitle |
E-cadherin and DAP kinase in pancreatic adenocarcinoma and corresponding lymph node metastases.
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pubmed:affiliation |
Department of Surgery, University of Leipzig, Liebigstr 20a, D-04103 Leipzig, Germany.
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pubmed:publicationType |
Journal Article
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