Linked Life Data

16595729

Source:http://linkedlifedata.com/resource/pubmed/id/16595729

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2006-4-5
pubmed:abstractText
The aim of our study was to test whether a reduction in dietary intake could improve in vitro embryo production in superovulated overfed dairy heifers. Cumulus-oocyte complexes of 16 Prim' Holstein heifers (14 +/- 1 months old) were collected by ovum pick-up (OPU), every 2 weeks following superovulation treatment with 250 microg FSH, before being matured and fertilized in vitro. Embryos were cultured in Synthetic Oviduct Fluid medium for 7 days. Heifers were fed with hay, soybean meal, barley, minerals and vitamins. From OPU 1 to 4 (period 1), all heifers received individually for 8 weeks a diet formulated for a 1000 g/day live-weight gain. From OPU 5 to 8 (period 2), the heifers were allocated to one of two diets (1000 or 600 g/day) for 8 weeks. Heifers' growth rates were monitored and plasma concentrations of metabolites, metabolic and reproductive hormones were measured each week. Mean live-weight gain observed during period 1 was 950 +/- 80 g/day (n = 16). In period 2 it was 730 +/- 70 (n = 8) and 1300 +/- 70 g/day (n = 8) for restricted and overfed groups respectively. When comparing period 1 and period 2 within groups, significant differences were found. In the restricted group, a higher blastocyst rate, greater proportions of grade 1-3 and grade 1 embryos, associated with higher estradiol at OPU and lower glucose and beta-hydroxybutyrate, were observed in period 2 compared with period 1. Moreover, after 6 weeks of dietary restriction (OPU 7), numbers of day 7 total embryos, blastocysts and grade 1-3 embryos had significantly increased. On the contrary, in the overfed group, we observed more <8 mm follicles 2 days before superovulation treatment, higher insulin and IGF-I and lower nonesterified fatty acids in period 2 compared with period 1 (no significant difference between periods for embryo production). After 6 weeks of 1300 g/day live-weight gain (OPU 7), embryo production began to decrease. Whatever the group, oocyte collection did not differ between period 1 and 2. These data suggest that following a period of overfeeding, a short-term dietary intake restriction (6 weeks in our study) may improve blastocyst production and embryo quality when they are low. However, nutritional recommendations aiming to optimize both follicular growth and embryonic development may be different.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1470-1626
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
783-94
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16595729-3-Hydroxybutyric Acid, pubmed-meshheading:16595729-Animal Nutritional Physiological Phenomena, pubmed-meshheading:16595729-Animals, pubmed-meshheading:16595729-Blastocyst, pubmed-meshheading:16595729-Blood Glucose, pubmed-meshheading:16595729-Body Weight, pubmed-meshheading:16595729-Cattle, pubmed-meshheading:16595729-Embryonic Development, pubmed-meshheading:16595729-Energy Intake, pubmed-meshheading:16595729-Fatty Acids, Nonesterified, pubmed-meshheading:16595729-Female, pubmed-meshheading:16595729-Fertilization in Vitro, pubmed-meshheading:16595729-Follicle Stimulating Hormone, pubmed-meshheading:16595729-Insulin, pubmed-meshheading:16595729-Insulin-Like Growth Factor I, pubmed-meshheading:16595729-Oogenesis, pubmed-meshheading:16595729-Ovarian Follicle, pubmed-meshheading:16595729-Superovulation, pubmed-meshheading:16595729-Time Factors, pubmed-meshheading:16595729-Urea
pubmed:year
2006
pubmed:articleTitle
Reduction of body-weight gain enhances in vitro embryo production in overfed superovulated dairy heifers.
pubmed:affiliation
UNCEIA Département Recherche et Développement, 13 rue Jouët, 94704 Maisons-Alfort cedex, France. sandrine.freret@unceia.fr
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't