Source:http://linkedlifedata.com/resource/pubmed/id/16594012
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-6-30
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| pubmed:abstractText |
We have measured directly the rate of formation of the oxidized chlorophyll a electron donor (P680(+)) and the reduced electron acceptor pheophytin a(-) (Pheoa(-)) following excitation of isolated spinach photosystem II reaction centers at 4 degrees C. The reaction-center complex consists of D(1), D(2), and cytochrome b-559 proteins and was prepared by a procedure that stabilizes the protein complex. Transient absorption difference spectra were measured from 440 to 850 nm as a function of time with 500-fs resolution following 610-nm laser excitation. The formation of P680(+)-Pheoa(-) is indicated by the appearance of a band due to P680(+) at 820 nm and corresponding absorbance changes at 505 and 540 nm due to formation of Pheoa(-). The appearance of the 820-nm band is monoexponential with tau = 3.0 +/- 0.6 ps. The time constant for decay of (1*)P680, the lowest excited singlet state of P680, monitored at 650 nm, is tau = 2.6 +/- 0.6 ps and agrees with that of the appearance of P680(+) within experimental error. Treatment of the photosystem II reaction centers with sodium dithionite and methyl viologen followed by exposure to laser excitation, conditions known to result in accumulation of Pheoa(-), results in formation of a transient absorption spectrum due to (1*)P680. We find no evidence for an electron acceptor that precedes the formation of Pheoa(-).
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16592698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16593659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16593728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16593792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16593899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-16666138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-17758561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-2819866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-3306679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-3527749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-3879185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-62,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16594012-6392571
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0027-8424
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
86
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
524-8
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1989
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| pubmed:articleTitle |
Determination of the primary charge separation rate in isolated photosystem II reaction centers with 500-fs time resolution.
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| pubmed:affiliation |
Chemistry Division, Argonne National Laboratory, Argonne, IL 60439.
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| pubmed:publicationType |
Journal Article
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