| pubmed-article:16593862 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0085245 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0331451 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0946575 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0029246 | lld:lifeskim |
| pubmed-article:16593862 | lifeskim:mentions | umls-concept:C0075739 | lld:lifeskim |
| pubmed-article:16593862 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:16593862 | pubmed:dateCreated | 2010-6-30 | lld:pubmed |
| pubmed-article:16593862 | pubmed:abstractText | Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system. | lld:pubmed |
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| pubmed-article:16593862 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16593862 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16593862 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16593862 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16593862 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:16593862 | pubmed:author | pubmed-author:HernalsteensJ... | lld:pubmed |
| pubmed-article:16593862 | pubmed:author | pubmed-author:De GreveHH | lld:pubmed |
| pubmed-article:16593862 | pubmed:author | pubmed-author:DeboeckFF | lld:pubmed |
| pubmed-article:16593862 | pubmed:author | pubmed-author:BytebierBB | lld:pubmed |
| pubmed-article:16593862 | pubmed:author | pubmed-author:MontaguM VMV | lld:pubmed |
| pubmed-article:16593862 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16593862 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:16593862 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16593862 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16593862 | pubmed:pagination | 5345-9 | lld:pubmed |
| pubmed-article:16593862 | pubmed:dateRevised | 2010-9-15 | lld:pubmed |
| pubmed-article:16593862 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:16593862 | pubmed:articleTitle | T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis. | lld:pubmed |
| pubmed-article:16593862 | pubmed:affiliation | Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium. | lld:pubmed |
| pubmed-article:16593862 | pubmed:publicationType | Journal Article | lld:pubmed |
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