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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2010-6-30
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pubmed:abstractText |
Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-11894957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-16453404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-16453482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-16453491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-16453585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-16593241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-3519133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6100893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6266929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6272339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6276020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6276681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-7108407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-7151163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-7253020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-7363328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-748950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593862-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5345-9
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pubmed:dateRevised |
2010-9-15
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pubmed:year |
1987
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pubmed:articleTitle |
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis.
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pubmed:affiliation |
Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium.
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pubmed:publicationType |
Journal Article
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