Source:http://linkedlifedata.com/resource/pubmed/id/16593831
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2010-6-30
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| pubmed:abstractText |
Tissue culture-derived mutants of male-sterile and disease toxin-sensitive Texas (T)-cytoplasm maize that exhibit male fertility and toxin insensitivity carry numerous alterations in mitochondrial DNA. In these mutants, a 6.7-kilobase Xho I fragment characteristic of parental T cytoplasm has been rearranged. In the mutant T-4, the parental 6.7-kilobase Xho I fragment contains a guanine to adenine transition adjacent to a 5-base-pair insertion not found in T cytoplasm. The insertion, internal to a 345-base-pair open reading frame (T ORF13), generates a frameshift, resulting in a premature stop codon that terminates the open reading frame at base pair 222. In other mutants, the 345-base-pair ORF is part of a 3-kilobase deletion, which extends into a 5-kilobase repeat characteristic of mtDNA from T but not N male-fertile cytoplasm. Clones specific to T ORF13 hybridize to eight transcripts in T and T-4, yet only hybridize to three transcripts in T-7, a deletion mutant. Transcription of the T ORF13 region appears not to be altered in T-4, but the frameshift mutation in the T ORF13 reading frame indicates that a biologically inactive gene product could be associated with the mutational events. The results suggest that cytoplasmic male sterility and disease toxin sensitivity may be associated with presence of T ORF13 in T-cytoplasm maize.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-16592467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-16592554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-16664516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-17248823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-17747315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-2417728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-6269087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-6364962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593831-6728934
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0027-8424
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
84
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2858-62
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| pubmed:dateRevised |
2010-9-15
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| pubmed:year |
1987
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| pubmed:articleTitle |
Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame.
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| pubmed:affiliation |
Department of Plant Pathology, University of Florida, Gainesville, FL 32611.
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| pubmed:publicationType |
Journal Article
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