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| pubmed-article:16593716 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:16593716 | pubmed:dateCreated | 2010-6-30 | lld:pubmed |
| pubmed-article:16593716 | pubmed:abstractText | We have developed a sensitive leaf disc transformation procedure for studying early and/or transient T-DNA expression during Agrobacterium tumefaciens-mediated transformation of plant cells. Using this system, we have examined the function of T-DNA border sequences on the early expression of T-DNA genes and on the stable integration of those genes in infected cells. Deletion of the right border from the T-DNA appears to permit transfer of T-DNA genes from the tumor-inducing (Ti) plasmid but greatly reduces the frequency of their stable integration. A binary vector has been constructed to permit examination of T-DNA border function in trans to the Ti plasmid. In this situation, a single T-DNA border is necessary for early expression of T-DNA genes and is sufficient for stable integration in any orientation. | lld:pubmed |
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| pubmed-article:16593716 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16593716 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16593716 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16593716 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16593716 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:16593716 | pubmed:author | pubmed-author:KleeH JHJ | lld:pubmed |
| pubmed-article:16593716 | pubmed:author | pubmed-author:HorschR BRB | lld:pubmed |
| pubmed-article:16593716 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16593716 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:16593716 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16593716 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16593716 | pubmed:pagination | 4428-32 | lld:pubmed |
| pubmed-article:16593716 | pubmed:dateRevised | 2010-9-15 | lld:pubmed |
| pubmed-article:16593716 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:16593716 | pubmed:articleTitle | Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process. | lld:pubmed |
| pubmed-article:16593716 | pubmed:affiliation | Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198. | lld:pubmed |
| pubmed-article:16593716 | pubmed:publicationType | Journal Article | lld:pubmed |
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