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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0035820,
umls-concept:C0075739,
umls-concept:C0085472,
umls-concept:C0205284,
umls-concept:C0242724,
umls-concept:C0348011,
umls-concept:C0439831,
umls-concept:C1510411,
umls-concept:C1510438,
umls-concept:C1517294,
umls-concept:C1522240,
umls-concept:C1556138,
umls-concept:C1705370,
umls-concept:C1705822
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pubmed:issue |
12
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pubmed:dateCreated |
2010-6-30
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pubmed:abstractText |
We have developed a sensitive leaf disc transformation procedure for studying early and/or transient T-DNA expression during Agrobacterium tumefaciens-mediated transformation of plant cells. Using this system, we have examined the function of T-DNA border sequences on the early expression of T-DNA genes and on the stable integration of those genes in infected cells. Deletion of the right border from the T-DNA appears to permit transfer of T-DNA genes from the tumor-inducing (Ti) plasmid but greatly reduces the frequency of their stable integration. A binary vector has been constructed to permit examination of T-DNA border function in trans to the Ti plasmid. In this situation, a single T-DNA border is necessary for early expression of T-DNA genes and is sufficient for stable integration in any orientation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-16453404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-16453483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-16453603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-16593241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-16593495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-17757866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-31918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-3458219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-3860847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-4044529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-4530328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6089175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6091129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6095209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6276020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6296058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6297376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6365544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6373732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6467373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6473100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-6839358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-7012838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-7108407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593716-7151163
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4428-32
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pubmed:dateRevised |
2010-9-15
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pubmed:year |
1986
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pubmed:articleTitle |
Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.
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pubmed:affiliation |
Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198.
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pubmed:publicationType |
Journal Article
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