Linked Life Data

16593620

Source:http://linkedlifedata.com/resource/pubmed/id/16593620

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2010-6-30
pubmed:abstractText
Increased DNA levels in centrally located endosperm nuclei are shown to be related to endosperm development in Zea mays. Mitotic activity sharply decreases in endosperm cells 10-12 days after pollination. At this time nuclear size and DNA content per nucleus (where C = haploid content) sharply increase until peak levels are reached at about 14-18 days after pollination. Mean DNA content per endosperm nucleus in strain A188 was shown by Feulgen cytophotometry to increase to about 90C by this peak stage, with the pattern being remarkably consistent over four consecutive growing seasons. Some individual nuclei achieved levels of >200C. Most other strains compared during one growing season averaged even higher peak levels of DNA per nucleus than did A188. Individual nuclei in those strains reached levels as high as 690C. A decrease in DNA level was observed in older endosperms with most strains. Endosperm mutant strains did not show a significant reduction in DNA. Opaque-2 mutants in several backgrounds achieved higher levels of DNA per nucleus. DNA levels from F(1) endosperms did not indicate heterosis. Regardless of differences in DNA content, the pattern of DNA increasing as development proceeds followed by a DNA decrease was observed for most strains. Cytological studies reveal much variation in chromatin strandedness, a maximum of three nucleoli, a maximum of three nucleolar organizer regions, and approximately 30 diffuse chromatin masses in older endosperm tissue. A form of DNA amplification, perhaps polytenization, appears to be occurring during endosperm development.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7010-4
pubmed:dateRevised
2010-9-15
pubmed:year
1985
pubmed:articleTitle
DNA amplification patterns in maize endosperm nuclei during kernel development.
pubmed:affiliation
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108.
pubmed:publicationType
Journal Article