Linked Life Data

1659212

Source:http://linkedlifedata.com/resource/pubmed/id/1659212

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1991-12-20
pubmed:abstractText
Rabbit corneas were mounted in Ussing chambers, and the apical membrane of the superficial cells (SCs) was permeabilized by exposure to digitonin in a Ca(2+)-free Ringer solution. This treatment resulted in the generation of large (60.7 +/- 13.2 microA/cm2, n = 25) Na(+)-dependent tear (T)-to-stroma (S) short-circuit currents (Isc). The Isc was abolished by ouabain and by 1.4 mM Ca2+ and was inhibited by heptanol, 18 alpha-glycyrrhetinic acid, and dieldrin, effects consistent with the notion that corneal transepithelial fluxes include translocations through gap junctions (GJs) before basolateral membrane transport. T-to-S Isc were also generated when T-side Na+ was replaced by K+, eliciting a T-to-S K+ flux via basolateral K+ channels and when, with either Na+ or K+ on the T side, channels were introduced at the apical membrane with amphotericin B. The Isc in all four conditions exhibited similar sensitivity to GJ inhibitors and were inhibited by adenosine 3',5'-cyclic monophosphate (cAMP) elevation. Fluorophotometry combined with SC permeabilization with digitonin demonstrated that the half-time for the SC to sub-SC movement of 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (mol wt 540) exceeded 3 h. These results indicate that junctional communications along the epithelial stratification axis are highly restricted and modulated by cAMP concentration.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
261
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C857-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Gap junctions in rabbit corneal epithelium: limited permeability and inhibition by cAMP.
pubmed:affiliation
Department of Ophthalmology, Mount Sinai School of Medicine, New York, New York 10029.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't