Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12 Pt 1-2
pubmed:dateCreated
2010-6-29
pubmed:abstractText
Brief irradiation of a buffer extract of dark-grown zucchini squash seedlings with red light results in the binding of the far-red-absorbing form of phytochrome to a particulate fraction. A low concentration of magnesium (0.1 mM) permits partial far-red reversal of the binding. A higher concentration (10 mM) yields actually enhanced binding after the far-red treatment. Both magnesium and calcium have a strong effect on the vesicularization of the phytochrome-binding particles and on their aggregation into readily sedimentable complexes. At concentrations above 10 mM, binding of the far-red-absorbing form of phytochrome is inhibited. These effects were not observed with sodium or potassium. Increasing the H(+) concentration led to increased binding of the far-red-absorbing form. This form of phytochrome bound at pH 6.5 and 10 mM magnesium is released if either the pH is raised to 8.0 or the magnesium concentration is raised to 50 mM. These properties suggest a new method for phytochrome purification.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3861-5
pubmed:dateRevised
2010-9-14
pubmed:year
1973
pubmed:articleTitle
Binding properties in vitro of phytochrome to a membrane fraction.
pubmed:affiliation
The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
pubmed:publicationType
Journal Article