Linked Life Data

1659059

Source:http://linkedlifedata.com/resource/pubmed/id/1659059

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-12-13
pubmed:abstractText
A Polyomavirus mutant with a 504 base pair deletion downstream of the polyadenylation signal for the early genes was constructed in vitro. This mutant showed a reduced synthesis of viral DNA. In cotransfection experiments, this defect was complemented by the presence of a wild-type genome. To define the sequences involved in the determination of this phenotype, a set of viral mutants was constructed. The properties of these mutants suggested that the deletion of a short DNA segment located 35 base pairs downstream of the early polyadenylation site affected the stability of early mRNA. The boundaries of the deletion were within the late coding sequences. However, the truncated form of the major capsid protein VP1 expressed by the mutant, did not influence the formation of early mRNA and the synthesis of viral DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-57
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Reduced Polyomavirus DNA replication as a consequence of a late-region deletion that results in early mRNA instability.
pubmed:affiliation
Dipartimento di Genetica e Biologia Molecolare, Università di Roma, La Sapienza, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't