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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1991-12-20
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pubmed:abstractText |
After elimination of the virus fraction non-associated with bovine leukemia virus (free) glycoprotein gp-51 antigen has been preparatively isolated from the supernatant of the FLK cell culture. For this purpose ultrafiltration through PM-30 membrane, thrice-repeated isoelectric focusing with different (pH 3.0-10.0, pH 4.0-6.0) ampholine intervals and affinity chromatography with ConA-sepharose are used. The SDS-polyacrylamide gel electrophoresis has determined it to be a homogeneous protein with molecular weight 51,000 daltons. The results of the enzyme-linked immunosorbent assay, immunoelectrophoresis and agar-gel immunodiffusion test confirm the specific activity of isolated gp-51 antigen. The gp-51 antigen may be used for identification of antibodies in the blood serum of leukemia virus of infected cattle and for monoclonal screening of antibodies.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0201-8470
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-36
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1659007-Animals,
pubmed-meshheading:1659007-Antigens, Viral,
pubmed-meshheading:1659007-Cell Line,
pubmed-meshheading:1659007-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1659007-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1659007-Immunodiffusion,
pubmed-meshheading:1659007-Isoelectric Focusing,
pubmed-meshheading:1659007-Leukemia Virus, Bovine,
pubmed-meshheading:1659007-Membrane Glycoproteins,
pubmed-meshheading:1659007-Sheep
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pubmed:articleTitle |
[Glycoprotein (gp-51) antigen not associated with the bovine leukemia virus in FLK culture supernatant liquid].
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pubmed:publicationType |
Journal Article,
English Abstract
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