Linked Life Data

16583200

Source:http://linkedlifedata.com/resource/pubmed/id/16583200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-5-31
pubmed:abstractText
Candida cylindracea NRRL Y-17506 was grown to produce extracellular lipase from oleic acid as a carbon source. Through flask cultures, it was found that the optimum initial oleic acid concentration for cell growth was 20 g l(-1). However, high initial concentrations of oleic acid up to 50 g l(-1) were not inhibitory. The highest extracellular lipase activity obtained in flask culture was 3.0 U ml(-1) after 48 h with 5 g l(-1) of initial oleic acid concentration. Fed-batch cultures (intermittent and stepwise feeding) were carried out to improve cell concentration and lipase activity. For the intermittent feeding fed-batch culture, the final cell concentration was 52 g l(-1) and the extracellular lipase activity was 6.3 U ml(-1) at 138.5 h. Stepwise feeding fed-batch cultures were carried out to simulate an exponential feeding and to investigate the effects of specific growth rate (0.02, 0.04 and 0.08 h(-1)) on cell growth and lipase production. The highest final cell concentration obtained was 90 g l(-1) when the set point of specific growth rate (mu(set)) was 0.02 h(-1). High specific growth rate (0.04 and 0.08 h(-1)) decreased extracellular lipase production in the later part of fed-batch cultures due to build-up of the oleic acid oversupplied. The highest extracellular lipase activity was 23.7 U ml(-1) when mu(set) was 0.02 h(-1), while the highest lipase productivity was 0.31 U ml(-1) h(-1) at mu(set) of 0.08 h(-1).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1615-7591
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Production of lipase by high cell density fed-batch culture of Candida cylindracea.
pubmed:affiliation
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't