rdf:type |
|
lifeskim:mentions |
umls-concept:C0010423,
umls-concept:C0043309,
umls-concept:C0073240,
umls-concept:C0086418,
umls-concept:C0439611,
umls-concept:C0936012,
umls-concept:C1309483,
umls-concept:C1333218,
umls-concept:C1514562,
umls-concept:C1707271,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
|
pubmed:issue |
Pt 4
|
pubmed:dateCreated |
2006-4-3
|
pubmed:abstractText |
Human Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C-terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group C222(1), with unit-cell parameters a = 88.6, b = 199.7, c = 119.6 angstroms, and diffracted X-rays to 2.0 angstroms resolution. The asymmetric unit contained three molecules of the RNase IIIb and the solvent content was 67%.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
1744-3091
|
pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:day |
1
|
pubmed:volume |
62
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
402-4
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
|
pubmed:year |
2006
|
pubmed:articleTitle |
Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer.
|
pubmed:affiliation |
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|