16581786

pubmed:Citation

8

MEF is an ETS-related transcription factor with strong transcriptional activating activity that affects hematopoietic stem cell behavior and is required for normal NK cell and NK T-cell development. The MEF (also known as ELF4) gene is repressed by several leukemia-associated fusion transcription factor proteins (PML-retinoic acid receptor alpha and AML1-ETO), but it is also activated by retroviral insertion in several cancer models. We have previously shown that cyclin A-dependent phosphorylation of MEF largely restricts its activity to the G(1) phase of the cell cycle; we now show that MEF is a short-lived protein whose expression level also peaks during late G(1) phase. Mutagenesis studies show that the rapid turnover of MEF in S phase is dependent on the specific phosphorylation of threonine 643 and serine 648 at the C terminus of MEF by cdk2 and on the Skp1/Cul1/F-box (SCF) E3 ubiquitin ligase complex SCF(Skp2), which targets MEF for ubiquitination and proteolysis. Overexpression of MEF drives cells through the G(1)/S transition, thereby promoting cell proliferation. The tight regulation of MEF levels during the cell cycle contributes to its effects on regulating cell cycle entry and cell proliferation.

http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-10207087, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-10531037, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-10559916, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-10611969, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-10998601, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-11331599, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-11463878, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-11504716, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-11533444, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-11565034, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12185367, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12234927, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12387738, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12435635, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12646216, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12769843, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12893766, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-12925736, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-1388288, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-14625302, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-15199159, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-15298723, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-15340381, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-15668399, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-15714552, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-7553852, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-7958856, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8087845, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8434021, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8493578, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8706131, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8769642, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8861947, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8895518, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-8895573, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-9122175, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-9311993, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-9346238, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-9529603, http://linkedlifedata.com/resource/pubmed/commentcorrection/16581786-9891053

http://linkedlifedata.com/resource/pubmed/journal/8109087

http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/ELF4 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins, http://linkedlifedata.com/resource/pubmed/chemical/Ligases, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Serine, http://linkedlifedata.com/resource/pubmed/chemical/Threonine, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin, http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylleucyl-leucyl-leuci...

Apr

pubmed-author:HedvatCyrus VCV, pubmed-author:KoffAndrewA, pubmed-author:LiuYanY, pubmed-author:MaoShifengS, pubmed-author:NguyenHoangH, pubmed-author:NimerStephen DSD, pubmed-author:YaoJinjuanJ, pubmed-author:ZhuXin-HuaXH

26

Y

2009-11-18