Linked Life Data

1657718

Source:http://linkedlifedata.com/resource/pubmed/id/1657718

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-12-13
pubmed:abstractText
The genomic structure of bovine syncytial virus (BSV), a virus commonly infecting cattle, was examined in order to gain insights into the nature of viral DNA (vDNA) intermediates and the transcriptional status of the virus in cytopathic infections. In dog Cf2Th cells, the DNA intermediate of BSV was found to exist predominantly as linear unintegrated vDNA (uvD) molecules. The uvD molecules were cloned directly from total cellular DNA by addition of EcoRI linkers and subsequent ligation into the phage lambda EMBL4 vector. Of the eleven clones characterized, seven were full length as judged by restriction fragment analysis. The remaining four clones showed varying degrees of heterogeneity in the form of internal deletions or terminal truncations. Heat denaturation and S1 nuclease analyses were used to show that vDNA isolated from Cf2Th cells contains a single-stranded (ss) gap structure located in the central region of the genome. In addition, a double-stranded (ds) 1.3-kb fragment is observed in this vDNA population. Northern-blot analysis revealed the presence of virus-specific transcripts of 11.0, 6.4, 2.8, and 2.4 kb. This suggests that BSV is similar in complexity to the lentiviruses in terms of linear intermediates containing ss gap structures, and the presence of several RNA transcripts which may direct complex regulatory functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Structure and transcriptional status of bovine syncytial virus in cytopathic infections.
pubmed:affiliation
Department of Microbiology, Immunology, and Parasitology, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't