Linked Life Data

16572345

Source:http://linkedlifedata.com/resource/pubmed/id/16572345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-4-21
pubmed:abstractText
We present here a new accessory for IR transmission measurements of 1H/2H exchange, as an ancillary tool for monitoring structural features of biomolecules in aqueous solution. This new accessory results from the combination of two dialysis membranes and a conventional liquid cell having two cylinders containing 2H2O buffer. When compared with conventional transmission measurements, carried out either after dissolving lyophilized biomolecules in 2H2O or after dialyzing the aqueous solution considered against 2H2O buffer, this accessory shows the following advantages: (1) controlled measurements over the initial steps of this isotopic exchange and absence of molecular aggregation, and (2) smaller sample amounts. This new Fourier transform IR cell can also be used to analyze ligand-biomolecule and drug-cell interactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1618-2642
pubmed:author
pubmed:issnType
Print
pubmed:volume
385
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
134-8
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
New accessory for studies of isotopic 1H/2H exchange and biomolecular interactions using transmission infrared spectroscopy.
pubmed:affiliation
Instituto de Estructura de la Materia (CSIC), Serrano 121, 28006, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't