rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
3
|
pubmed:dateCreated |
1991-11-1
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pubmed:databankReference |
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pubmed:abstractText |
DNA from a rat hippocampus cDNA library and sets of highly degenerate oligonucleotide primers directed toward conserved regions of previously cloned G-protein receptors were used in the polymerase chain reaction to selectively amplify and clone new members of this gene family. A human hippocampus cDNA library was screened with a 610 base pair fragment generated by PCR and a cDNA clone, H318/3, was isolated. The deduced amino acid sequence of this clone encoded a protein of 501 amino acids that showed strong sequence homology to previously cloned G-protein receptors. Nucleotide sequence analysis revealed clone H318/3 was 78% homologous to a rat alpha 1A adrenergic receptor with homology being 95% when comparisons were made in the region that lies between the first to the seventh transmembrane domains. Based on this high degree of sequence homology, we conclude that clone H318/3 represents a cDNA for a human alpha 1A adrenergic receptor.
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pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0006-291X
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
30
|
pubmed:volume |
179
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1485-90
|
pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1656955-Amino Acid Sequence,
pubmed-meshheading:1656955-Animals,
pubmed-meshheading:1656955-Base Sequence,
pubmed-meshheading:1656955-DNA,
pubmed-meshheading:1656955-Gene Library,
pubmed-meshheading:1656955-Hippocampus,
pubmed-meshheading:1656955-Humans,
pubmed-meshheading:1656955-Molecular Sequence Data,
pubmed-meshheading:1656955-Oligodeoxyribonucleotides,
pubmed-meshheading:1656955-Polymerase Chain Reaction,
pubmed-meshheading:1656955-Rats,
pubmed-meshheading:1656955-Receptors, Adrenergic, alpha,
pubmed-meshheading:1656955-Sequence Homology, Nucleic Acid
|
pubmed:year |
1991
|
pubmed:articleTitle |
Molecular cloning and sequencing of a cDNA encoding a human alpha 1A adrenergic receptor.
|
pubmed:affiliation |
Department of Medicine, State University of New York, Stony Brook 11794.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|