Linked Life Data

16569201

Source:http://linkedlifedata.com/resource/pubmed/id/16569201

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-3-29
pubmed:abstractText
BACKGROUND: Lymphatic vascular endothelial hyaluronan receptor-1 (LYVE-1) is a selective marker for lymphatic endothelium and a homolog of CD44, the hyaluronan (HA) receptor. HA in the extracellular matrix plays roles in tissue remodeling, development, and homeostasis, and as an HA receptor, LYVE-1 mediated HA metabolism might regulate these events. Currently, little is known about the lymphatic character within the human placenta. This study therefore determined LYVE-1 and other lymphatic markers in human placentas. METHODS AND RESULTS: Placentas and villous tissue were fixed and immunostained for human LYVE-1 and CD44 and examined by RT-PCR. LYVE-1 was expressed at both protein and mRNA levels in trophoblast cells (TC) and in villous core endothelium (VCE). Predominant protein expression for LYVE-1 was observed in syncytiotrophoblast cells (TCs) of preterm placentas. Neither mRNA or protein for CD44 was expressed. Other blood and lymphatic-lineage molecules (VEGF-A, -C, and -D, Flt-1, KDR, Flt-4, and Prox-1) were examined by RT-PCR. VEGF-A, VEGF-D, and Flt-1 mRNA were observed in TCs and VCEs, while mRNA for VEGF-C, KDR, and Flt-4 was mainly observed in VCEs. Prox-1 was found at the mRNA, but not protein level in TCs and VCEs. Our findings indicate (1) the importance of LYVE-1, but not CD44, in regulation of HA metabolism in the maternal-fetal interface and fetal circulation, and (2) possible dual blood and lymphatic phenotypic characteristics in fetal endothelium. These results provide new insights into HA metabolism and lymphatic-lineage molecule expression in the human placenta.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-10037799, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-10978893, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-11170825, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-11278811, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-11332691, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-11943725, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-14676121, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-15257549, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-3905758, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-7691670, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-7937593, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-8379464, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-8592064, http://linkedlifedata.com/resource/pubmed/commentcorrection/16569201-8980911
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1539-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-7
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Expression of lymphatic vascular endothelial hyaluronan receptor-1 (LYVE-1) in the human placenta.
pubmed:affiliation
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, 71130, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural