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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-6-5
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pubmed:abstractText |
The Wnt-signaling pathway plays a critical role in directing cell fate during embryogenesis. Several lines of evidence also suggest a role in inflammatory processes. Here, we analyzed whether Wnt signaling plays a role in leukocyte inflammatory responses. Monocytes from healthy donors expressed different Frizzled receptors, which are ligands for the Wnt molecules. Activation of the Wnt/beta-catenin pathway by LiCl or Wnt3a increased beta-catenin protein levels in monocytes but not in granulocytes. It is interesting that the activation of Wnt/beta-catenin signaling via Wnt3a in monocytes resulted in a decrease in migration through an endothelial layer (human dermal microvascular endothelial cell-1). Further experiments revealed that the decrease in transendothelial migration was associated with specific monocyte adherence to endothelial cells after Wnt exposure. The specificity was verified by a lack of Wnt3a-induced adhesion to fibronectin, laminin, or collagen compared with endothelial interaction. Analysis of the distribution of beta-catenin revealed a Wnt3a-induced increase of beta-catenin in the cytoplasm. Wnt3a exposure did not result in any activation of the classical Wnt-target gene c-myc or a Wnt-target gene involved in cell adhesion (Connexin43). Our study implicates for the first time a role of canonical Wnt signaling in inflammatory processes in monocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Frizzled Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Lithium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/WNT3A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3A Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt3a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0741-5400
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pubmed:author |
pubmed-author:BaasMarionM,
pubmed-author:BerdelWolfgang EWE,
pubmed-author:ChoudharyChunaramC,
pubmed-author:GerkeVolkerV,
pubmed-author:HehnSinaS,
pubmed-author:Müller-TidowCarstenC,
pubmed-author:SarginBülentB,
pubmed-author:SchwäbleJoachimJ,
pubmed-author:ServeHubertH,
pubmed-author:StreyAnkeA,
pubmed-author:TickenbrockLaraL
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1306-13
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16565323-Animals,
pubmed-meshheading:16565323-Cell Adhesion,
pubmed-meshheading:16565323-Cell Differentiation,
pubmed-meshheading:16565323-Cell Movement,
pubmed-meshheading:16565323-Cell Nucleus,
pubmed-meshheading:16565323-Cells, Cultured,
pubmed-meshheading:16565323-Cytoplasm,
pubmed-meshheading:16565323-Endothelium, Vascular,
pubmed-meshheading:16565323-Frizzled Receptors,
pubmed-meshheading:16565323-Granulocytes,
pubmed-meshheading:16565323-HL-60 Cells,
pubmed-meshheading:16565323-Humans,
pubmed-meshheading:16565323-Lithium Chloride,
pubmed-meshheading:16565323-Mice,
pubmed-meshheading:16565323-Monocytes,
pubmed-meshheading:16565323-Recombinant Fusion Proteins,
pubmed-meshheading:16565323-Signal Transduction,
pubmed-meshheading:16565323-Wnt Proteins,
pubmed-meshheading:16565323-Wnt3 Protein,
pubmed-meshheading:16565323-Wnt3A Protein,
pubmed-meshheading:16565323-beta Catenin
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pubmed:year |
2006
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pubmed:articleTitle |
Wnt signaling regulates transendothelial migration of monocytes.
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pubmed:affiliation |
Department of Medicine, Hematology and Oncology, University of Münster, Domagkstr. 3, 48129 Münster, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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