Linked Life Data

1656316

Source:http://linkedlifedata.com/resource/pubmed/id/1656316

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-11-15
pubmed:abstractText
Preproenkephalin metabolism, in the rat, was studied in primary striatal neurons maintained in a chemically defined medium. Acute treatment (30 min) with forskolin (10(-5) M) or phorbol 12 myristate 13 acetate (10(-7) M) resulted, respectively, in a two- and seven-fold increase in methionine-enkephalin secretion. Chronic treatment with forskolin or phorbol 12 myristate 13 acetate (24 h) induced a 100% increase in methionine-enkephalin content (forskolin) and on the other hand a 50% decrease in methionine-enkephalin (phorbol 12 myristate 13 acetate). Both treatments increased preproenkephalin mRNA levels in a time-dependent manner, this augmentation being observable after 180 min by Northern blot analysis and in situ hybridization. These data indicate that under chronic stimulation, with either forskolin or phorbol 12 myristate 13 acetate, proenkephalin turnover is accelerated. However, after stimulation with phorbol 12 myristate 13 acetate, the more potent methionine-enkephalin secretagogue, increased peptide synthesis is not sufficient to replenish methionine-enkephalin intracellular stores. Preproenkephalin gene transcription was analysed by introducing the preproenkephalin gene promoter fused to the bacterial acetyl chloramphenicol transferase reporter gene into primary neurons. Chronic stimulation (48 h) by forskolin (10(-5) M) or phorbol 12 myristate 13 acetate (10(-7) M) of striatal neurons transfected with this fusion gene increased chloramphenicol acetyltransferase activity six-fold and the two effects were additive. These data suggest that the cyclic AMP and the protein kinase C pathways directly activate preproenkephalin gene transcription.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-79
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1656316-Animals, pubmed-meshheading:1656316-Cells, Cultured, pubmed-meshheading:1656316-Corpus Striatum, pubmed-meshheading:1656316-Cyclic AMP, pubmed-meshheading:1656316-Enkephalin, Methionine, pubmed-meshheading:1656316-Enkephalins, pubmed-meshheading:1656316-Female, pubmed-meshheading:1656316-Forskolin, pubmed-meshheading:1656316-Immunohistochemistry, pubmed-meshheading:1656316-Neural Pathways, pubmed-meshheading:1656316-Neurons, pubmed-meshheading:1656316-Nucleic Acid Hybridization, pubmed-meshheading:1656316-Pregnancy, pubmed-meshheading:1656316-Protein Kinase C, pubmed-meshheading:1656316-Protein Precursors, pubmed-meshheading:1656316-RNA, Messenger, pubmed-meshheading:1656316-Rats, pubmed-meshheading:1656316-Rats, Inbred Strains, pubmed-meshheading:1656316-Receptors, Neurotransmitter, pubmed-meshheading:1656316-Tetradecanoylphorbol Acetate, pubmed-meshheading:1656316-Transcription, Genetic, pubmed-meshheading:1656316-Transfection
pubmed:year
1991
pubmed:articleTitle
Striatal proenkephalin turnover and gene transcription are regulated by cyclic AMP and protein kinase C-related pathways.
pubmed:affiliation
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U297 affilée au CRNS, Faculté de Médecine Nord, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't