Source:http://linkedlifedata.com/resource/pubmed/id/16562154
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
When washed yeast cells grown under appropriate conditions were suspended in glucose solution there was a sudden release of alpha-amino nitrogen into the medium. This released material was of low molecular weight, and its composition was closely similar to that of the intracellular free amino acid pool. During the leakage of amino acids, the yeast did not efficiently absorb labeled amino acids added to the test medium, despite the rapid uptake and metabolism of glucose. Uptake of a labeled amino acid and reabsorption of the released alpha-amino nitrogen occurred almost simultaneously. When these yeast cells were exposed to glucose in the presence of calcium ions, leakage was strongly inhibited. Butanol under the same conditions increased glucose-induced leakage of cell contents. The adenosine triphosphatase activity of intact yeast cells exposed to glucose was greater than that of cells exposed to water. Yeast cells treated with glucose prior to equilibration with sorbose exhibited less ability to retain the sorbose when washed at 0 C than did cells pretreated with water. It was concluded that glucose-induced leakage of amino acids was the result of two factors acting together. These were (i) a change in membrane permeability associated with glucose uptake, and (ii) a temporary shortage of energy for amino acid uptake or retention.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-13221596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-13802308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-13816916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-13978645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14102863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14179673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14188718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14188768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14255711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14276122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16562154-14413698
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0021-9193
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
93
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
976-84
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1967
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| pubmed:articleTitle |
Glucose-induced Release of Amino Acids from Saccharomyces carlsbergensis by Action on the Cytoplasmic Membrane.
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| pubmed:affiliation |
Department of Food Science and Technology, University of California, Davis, California.
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| pubmed:publicationType |
Journal Article
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