1656085

Source:http://linkedlifedata.com/resource/pubmed/id/1656085

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0017262, umls-concept:C0035668, umls-concept:C0205145, umls-concept:C0206419, umls-concept:C0441587, umls-concept:C0851285, umls-concept:C1171362, umls-concept:C1274040, umls-concept:C1515670, umls-concept:C1622984, umls-concept:C2603343
pubmed:issue	11
pubmed:dateCreated	1991-11-14
pubmed:abstractText	A system that exploits defective interfering (DI) RNAs of mouse hepatitis virus (MHV) for deciphering the mechanisms of coronavirus mRNA transcription was developed. A complete cDNA clone of MHV DI RNA containing an inserted intergenic region, derived from the area of genomic RNA between genes 6 and 7, was constructed. After transfection of the in vitro-synthesized DI RNA into MHV-infected cells, replication of genomic DI RNA as well as transcription of the subgenomic DI RNA was observed. S1 nuclease protection experiments, sequence analysis, and Northern (RNA) blotting analysis revealed that the subgenomic DI RNA contained the leader sequence at its 5' end and that the body of the subgenomic DI RNA started from the inserted intergenic sequence. Two subgenomic DI RNAs were synthesized after inserting two intergenic sites into the MHV DI RNA. Metabolic labeling of virus-specific protein in DI RNA replicating cells demonstrated that a protein was translated from the subgenomic DI RNA, which can therefore be considered a functional mRNA. Transfection study of gel-purified genomic DI RNA and subgenomic DI RNA revealed that the introduction of the genomic DI RNA, but not subgenomic DI RNA, into MHV-infected cells was required for synthesis of the subgenomic DI RNA. A series of deletion mutations in the intergenic site demonstrated that the sequence flanking the consensus sequence of UCUAAAC affected the efficiency of subgenomic DI RNA transcription and that the consensus sequence was necessary but not sufficient for the synthesis of the subgenomic DI RNA.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/AI29984
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-1846489, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-1985203, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2033672, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-207885, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2154591, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2174358, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2243386, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2252386, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-228084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2538033, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2546161, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2547994, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2555555, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2556847, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2585607, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2823261, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2824826, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2831651, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2843681, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2845661, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2985802, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-2992183, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-3012558, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-3027981, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-4365902, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6093379, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6169842, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6245243, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6292513, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6304334, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6308569, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6322437, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-6328522, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-7281517, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-73185, http://linkedlifedata.com/resource/pubmed/commentcorrection/1656085-81487
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0113724
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral, http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements, http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	0022-538X
pubmed:author	pubmed-author:JoySS, pubmed-author:MakinoJ KJK, pubmed-author:MakinoSS
pubmed:issnType	Print
pubmed:volume	65
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	6031-41
pubmed:dateRevised	2009-11-18
pubmed:meshHeading	pubmed-meshheading:1656085-Animals, pubmed-meshheading:1656085-Base Sequence, pubmed-meshheading:1656085-Cell Line, pubmed-meshheading:1656085-Coronaviridae, pubmed-meshheading:1656085-DNA, Viral, pubmed-meshheading:1656085-DNA Transposable Elements, pubmed-meshheading:1656085-Defective Viruses, pubmed-meshheading:1656085-Genes, Viral, pubmed-meshheading:1656085-Genetic Techniques, pubmed-meshheading:1656085-Introns, pubmed-meshheading:1656085-Mice, pubmed-meshheading:1656085-Molecular Sequence Data, pubmed-meshheading:1656085-Murine hepatitis virus, pubmed-meshheading:1656085-Mutagenesis, Site-Directed, pubmed-meshheading:1656085-Oligodeoxyribonucleotides, pubmed-meshheading:1656085-Protein Biosynthesis, pubmed-meshheading:1656085-RNA, Messenger, pubmed-meshheading:1656085-RNA, Viral, pubmed-meshheading:1656085-Restriction Mapping, pubmed-meshheading:1656085-Sequence Homology, Nucleic Acid, pubmed-meshheading:1656085-Transcription, Genetic
pubmed:year	1991
pubmed:articleTitle	A system for study of coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion.
pubmed:affiliation	Department of Microbiology, University of Texas, Austin 78712.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.