rdf:type |
|
lifeskim:mentions |
umls-concept:C0007009,
umls-concept:C0009359,
umls-concept:C0013812,
umls-concept:C0013846,
umls-concept:C0017735,
umls-concept:C0068361,
umls-concept:C0086296,
umls-concept:C0439851,
umls-concept:C0522500,
umls-concept:C1450054,
umls-concept:C1552596,
umls-concept:C1704608,
umls-concept:C1947931
|
pubmed:issue |
2
|
pubmed:dateCreated |
2006-10-23
|
pubmed:abstractText |
The direct electron transfer of glucose oxidase (GOD) was achieved based on the immobilization of GOD/colloidal gold nanoparticles on a glassy carbon electrode by a Nafion film. The immobilized GOD displayed a pair of well-defined and nearly reversible redox peaks with a formal potential (Eo ') of -0.434 V in 0.1 M pH 7.0 phosphate buffer solution and the response showed a surface-controlled electrode process. The dependence of Eo ' on solution pH indicated that the direct electron transfer reaction of GOD was a two-electron-transfer coupled with a two-proton-transfer reaction process. The experimental results also demonstrated that the immobilized GOD retained its electrocatalytic activity for the oxidation of glucose. So the resulting modified electrode can be used as a biosensor for detecting glucose.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
1567-5394
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
69
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
158-63
|
pubmed:meshHeading |
pubmed-meshheading:16556513-Biosensing Techniques,
pubmed-meshheading:16556513-Carbon,
pubmed-meshheading:16556513-Catalysis,
pubmed-meshheading:16556513-Electrochemistry,
pubmed-meshheading:16556513-Electron Transport,
pubmed-meshheading:16556513-Enzymes, Immobilized,
pubmed-meshheading:16556513-Fluorocarbon Polymers,
pubmed-meshheading:16556513-Glucose,
pubmed-meshheading:16556513-Glucose Oxidase,
pubmed-meshheading:16556513-Gold Colloid,
pubmed-meshheading:16556513-Microelectrodes,
pubmed-meshheading:16556513-Nanoparticles,
pubmed-meshheading:16556513-Particle Size
|
pubmed:year |
2006
|
pubmed:articleTitle |
Glucose oxidase/colloidal gold nanoparticles immobilized in Nafion film on glassy carbon electrode: Direct electron transfer and electrocatalysis.
|
pubmed:affiliation |
College of Chemistry, Jilin University, Changchun, 130012, PR China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|