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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 1
|
| pubmed:dateCreated |
1991-10-31
|
| pubmed:abstractText |
Three oligonucleotide primer combinations selected from the 5' noncoding, the nucleocapsid and the putative nonstructural regions of the hepatitis C virus genome were compared in a nested polymerase chain reaction assay with respect to sensitivity and specificity for the detection of viral RNA in chimpanzee-infected and human-infected sera. Sera from both the acute and the chronic phase of the infection were obtained from 13 animals inoculated with five different non-A, non-B hepatitis strains and from seven cardiac surgery patients who had non-A, non-B hepatitis develop after transfusion and who had been tested in parallel for the presence of hepatitis C virus RNA and anti-C 100-3. A total of 90% of the acute-phase and 100% of the chronic-phase sera tested positive for hepatitis C virus RNA when the 5' noncoding-derived or the nucleocapsid-derived combinations were used; only 58% and 56%, respectively, gave positive results with the putative nonstructural primers, whereas 33% and 71%, respectively, scored positive for C100-3. Thus polymerase chain reaction primers selected from either the highly conserved 5' noncoding or nucleocapsid-regions appear to provide the sensitivity and the specificity necessary to detect low levels of hepatitis C virus RNA in both chimpanzee-infected and human-infected sera.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0270-9139
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
595-600
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:1655606-Animals,
pubmed-meshheading:1655606-Base Sequence,
pubmed-meshheading:1655606-Genome, Viral,
pubmed-meshheading:1655606-Hepacivirus,
pubmed-meshheading:1655606-Hepatitis C,
pubmed-meshheading:1655606-Molecular Sequence Data,
pubmed-meshheading:1655606-Oligonucleotide Probes,
pubmed-meshheading:1655606-Plasmids,
pubmed-meshheading:1655606-Polymerase Chain Reaction,
pubmed-meshheading:1655606-RNA, Viral,
pubmed-meshheading:1655606-Sensitivity and Specificity,
pubmed-meshheading:1655606-Viremia
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Use of conserved sequences from hepatitis C virus for the detection of viral RNA in infected sera by polymerase chain reaction.
|
| pubmed:affiliation |
New York Blood Center, Laboratory of Virology and Parasitology New York 10021.
|
| pubmed:publicationType |
Journal Article
|