Linked Life Data

1655185

Source:http://linkedlifedata.com/resource/pubmed/id/1655185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1991-11-8
pubmed:abstractText
Electron microscopy showed that electron-dense granules accumulated in Propionibacterium acnes in larger amounts when the bacteria were grown on a phosphate-rich medium. X-ray microanalysis demonstrated that the granules contained mostly phosphorus and potassium, indicating that the cells contained polyphosphate granules. When cells were grown on a complex Bacto-agar medium, the amount and the size of the polyphosphate granules were reduced. Polyphosphate was also detected with 31P nuclear magnetic resonance (31P-NMR). Of the polyphosphates observed with 31P-NMR, 20% seemed to be located outside the cell membrane. Broad-band near-ultraviolet irradiation (emission maximum 366 nm) corresponding to doses that killed 37% of the cells increased the amount of polyphosphate in cells grown on the phosphate-rich medium. The fluorescent chromophore 4',6-diamidino-2-phenylindole (DAPI) shifted the fluorescence emission from 478 to 538 nm when bound to polyphosphate and excited at 340 nm. DAPI was used to detect polyphosphates generated after near-ultraviolet irradiation of the cells. Nonirradiated cells showed no increased fluorescence at 538 nm, indicating no polyphosphate is presented in the cells. We conclude that DAPI did not have "access" to the intracellular polyphosphate as long as the cells were not light damaged. This observation is important for the interpretation of near-UV damage to cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0008-4166
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
562-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Changes in polyphosphate composition and localization in Propionibacterium acnes after near-ultraviolet irradiation.
pubmed:affiliation
Department of Physics, University of Trondheim, Dragvoll, Norway.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't