Source:http://linkedlifedata.com/resource/pubmed/id/16547768
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2006-7-5
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| pubmed:abstractText |
RNA interference (RNAi) is the repression of gene expression through a cellular mechanism of transcript-specific mRNA degradation. RNAi has been observed in human cells and applied to the modulation of a variety of human transcripts. Our goals were to deliver small interfering RNA (siRNA) using a liposome-based method, and to show Bcr-Abl siRNA specificity against K-562 cells, alone or in combination with Gleevec. Both synthetic (syn) siRNA, consisting of homogeneous 21-nucleotide-long RNA duplexes specific for the Bcr-Abl fusion site, and recombinant (r)-generated Bcr-Abl siRNA were employed. siRNA was transfected into K-562 cells with greater than 90% efficiency using RNAiFect, as judged by fluorescence analysis. The Bcr-Abl transcript was inhibited using either siRNA preparation as measured by RT-PCR or real-time PCR. The IC(50) of Gleevec in the K-562 subline F(1) was lowered over 3-fold from 0.2 to 0.06 muM in cells transfected with either syn or rBcr-Abl siRNA. No effect was observed in cells after transfection with an irrelevant control siRNA. Therefore, K-562 cells transfected with RNAifect deliver Bcr-Abl siRNA efficiently and the Bcr-Abl siRNA decreased the IC(50) of Gleevec required to inhibit the high levels of Bcr-Abl protein found in K-562 cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Fusion Proteins, bcr-abl,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrimidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/imatinib
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
1021-7770
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
499-507
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16547768-Base Sequence,
pubmed-meshheading:16547768-Blotting, Western,
pubmed-meshheading:16547768-Cell Line, Tumor,
pubmed-meshheading:16547768-Cell Proliferation,
pubmed-meshheading:16547768-DNA Primers,
pubmed-meshheading:16547768-Dose-Response Relationship, Drug,
pubmed-meshheading:16547768-Fusion Proteins, bcr-abl,
pubmed-meshheading:16547768-Humans,
pubmed-meshheading:16547768-Immunoprecipitation,
pubmed-meshheading:16547768-Inhibitory Concentration 50,
pubmed-meshheading:16547768-Liposomes,
pubmed-meshheading:16547768-Microscopy, Fluorescence,
pubmed-meshheading:16547768-Molecular Sequence Data,
pubmed-meshheading:16547768-Piperazines,
pubmed-meshheading:16547768-Pyrimidines,
pubmed-meshheading:16547768-RNA, Small Interfering,
pubmed-meshheading:16547768-RNA Interference,
pubmed-meshheading:16547768-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16547768-Sequence Analysis, DNA,
pubmed-meshheading:16547768-Transfection
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| pubmed:year |
2006
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| pubmed:articleTitle |
Effects of siRNAs in combination with Gleevec on K-562 cell proliferation and Bcr-Abl expression.
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| pubmed:affiliation |
Department of Medical Genetics, University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Box U-2, Knoxville, TN 37920, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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