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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-4-20
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pubmed:abstractText |
Infection of human foreskin fibroblast (HFF) cells with human cytomegalovirus (HCMV) induces the secretion of soluble factors including interferon (IFN)-beta that stimulates human leukocyte antigen (HLA) class I expression. In this study, the mechanism of IFN-beta induction by HCMV was investigated. In HCMV-infected HFF cells, IFN-beta secretion increased at 6h post infection (h.p.i.). Reverse transcription polymerase chain reaction (RT-PCR) analysis using ultra violet (UV)-inactivated HCMV indicated that viral gene expression is not necessary for the stimulation of IFN-beta. Stimulation of IFN-beta by HCMV infection was not blocked by cycloheximide, an inhibitor of protein synthesis, further suggesting that the expression of HCMV genes is not required for the stimulation of IFN-beta gene transcription. IFN-beta may be produced from virus-infected cells as an inflammatory response and nuclear factor kappa B (NF-kappaB) plays a central role in inflammatory response. HCMV failed to induce the IFN-beta expression, when the virus-infected cells were treated with pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kappaB, or LY294002 and wortmannin, inhibitors of phosphatidylinositol 3-kinase (PI3-K). The result suggests that PI3-K and/or NF-kappaB may be related with the induction pathway of IFN-beta by HCMV.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates,
http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0168-1702
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-14
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16545883-Androstadienes,
pubmed-meshheading:16545883-Cell Line,
pubmed-meshheading:16545883-Cycloheximide,
pubmed-meshheading:16545883-Cytomegalovirus,
pubmed-meshheading:16545883-Enzyme Inhibitors,
pubmed-meshheading:16545883-Fibroblasts,
pubmed-meshheading:16545883-Gene Expression,
pubmed-meshheading:16545883-Humans,
pubmed-meshheading:16545883-Interferon-beta,
pubmed-meshheading:16545883-NF-kappa B,
pubmed-meshheading:16545883-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:16545883-Protein Synthesis Inhibitors,
pubmed-meshheading:16545883-Pyrrolidines,
pubmed-meshheading:16545883-RNA, Messenger,
pubmed-meshheading:16545883-RNA, Viral,
pubmed-meshheading:16545883-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16545883-Thiocarbamates,
pubmed-meshheading:16545883-Transcription, Genetic
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pubmed:year |
2006
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pubmed:articleTitle |
Stimulation of interferon-beta gene expression by human cytomegalovirus via nuclear factor kappa B and phosphatidylinositol 3-kinase pathway.
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pubmed:affiliation |
Division of Life Sciences, College of Natural Sciences, and Biotechnology Research Institute, Chungbuk National University, 12 Gaeshindong, Cheongju, Chungbuk 361-763, South Korea. chlee@chungbuk.ac.kr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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