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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1991-10-21
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pubmed:databankReference |
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pubmed:abstractText |
A 13,863-base-pair (bp) putative centromeric DNA fragment has been isolated from a human genomic library by using a probe obtained from metaphase chromosomes of human colon carcinoma cells. The abundance of this DNA was estimated to be 16-32 copies per genome. Cotransfection of mouse cells with this sequence and a selectable marker gene (aminoglycoside 3'-phosphotransferase type II, APH-II) resulted in a transformed cell line carrying an additional centromere in a dicentric chromosome. This centromere was capable of binding an anti-centromere antibody. In situ hybridization demonstrated that the human DNA sequence as well as the APH-II gene and vector DNA sequences were located only in the additional centromere of the dicentric chromosome. The extra centromere separated from the dicentric chromosome, forming a stable minichromosome. This functional centromere linked to a dominant selectable marker may be a step toward the construction of an artificial mammalian chromosome.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-2541922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-2651051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-2830981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-2883581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-2994966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-3033825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-3057382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-322278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-3365767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-3458254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-3909945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-4130093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6271971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6350893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6383193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-6575390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-7196313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-719759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654558-7273954
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
8106-10
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1654558-Animals,
pubmed-meshheading:1654558-Centromere,
pubmed-meshheading:1654558-Cloning, Molecular,
pubmed-meshheading:1654558-DNA,
pubmed-meshheading:1654558-Humans,
pubmed-meshheading:1654558-Kanamycin Kinase,
pubmed-meshheading:1654558-Mice,
pubmed-meshheading:1654558-Molecular Sequence Data,
pubmed-meshheading:1654558-Nucleic Acid Hybridization,
pubmed-meshheading:1654558-Phosphotransferases,
pubmed-meshheading:1654558-Restriction Mapping
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pubmed:year |
1991
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pubmed:articleTitle |
Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene.
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pubmed:affiliation |
Institute of Genetics Biological Research Center, Hungarian Academy of Sciences, Szeged.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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