Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1991-10-22
pubmed:abstractText
The genome of a temperature-sensitive, DNA-negative mutant of human cytomegalovirus was cloned in cosmids and analyzed by restriction endonuclease mapping and Southern blotting. The data presented show that in the mutant genome, nearly half of the short segment was deleted (14.3 to 15.1 kb; map position, 0.83 to 0.9), including the genes for a potential immediate early protein (US3) and a structural glycoprotein of 47 to 52 kDa (US6 through US11). The deleted DNA region was replaced by a 20.8- to 21.6-kb fragment that represented an inverted repetition of the retained portion of the short segment (map position, 0.92 to 1.0), suggesting that US20 through US36 were duplicated in the mutant. Northern (RNA) blots with appropriate probes of total cell RNA extracted from mutant-infected cells confirmed the absence of mRNAs originating from US3 or from US8 through US11. It is concluded that the deleted genes are dispensable for human cytomegalovirus replication in cell culture.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-197204, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-198584, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2154887, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2161319, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2164376, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2171222, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-220788, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2552148, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2823255, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2829427, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2831396, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2833603, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2835500, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2842270, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2844970, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-2983334, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-3018842, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-3031311, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-3033823, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-3033890, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-6260575, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-6286417, http://linkedlifedata.com/resource/pubmed/commentcorrection/1654438-6310864
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5184-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
A 15-kilobase-pair region of the human cytomegalovirus genome which includes US1 through US13 is dispensable for growth in cell culture.
pubmed:affiliation
Institut für Virologie, Philipps-Universität, Marburg, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't