Linked Life Data

16543916

Source:http://linkedlifedata.com/resource/pubmed/id/16543916

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-7-19
pubmed:abstractText
Previous studies have shown that the human melanoma differentiation-associated gene-7 (mda-7)/interleukin-24 (IL-24) has tumor-suppressor activity in vitro and in vivo. Additionally, in vitro studies using human peripheral blood mononuclear cells indicate that mda-7/IL-24 has TH1 cytokine-like activity. However, the individual properties of mda-7/IL-24 have been previously examined separately. Thus, there is not a single study that has examined both, antitumor and proimmune properties of mda-7/IL-24. Furthermore, the tumor suppressive activity and the cytokine activity of mda-7/IL-24 have not been previously tested in an immunocompetent setting. We therefore in the present study evaluated the antitumor and immune properties of mda-7/IL-24 in a murine syngeneic tumor model. In vitro, adenovirus-mediated mda-7 gene (Ad-mda7) transfer to murine fibrosarcoma (UV2237m; MCA16) and normal (10T1/2) cells significantly inhibited growth (P=0.001) and induced apoptosis in tumor cells but not in normal cells. In vivo, intratumoral administration of Ad-mda7 resulted in significant inhibition of tumor growth (P<0.05), with a subset of mice showing complete tumor regression. We next evaluated the immune potentiation activity of Ad-mda7 in a cancer vaccine model. UV2237m cells transfected with Ad-mda7 and injected into syngeneic immunocompetent C3H mice were unable to grow; however, they did grow in immunocompromised nude mice. These tumor-free C3H mice, when challenged with parental tumor cells experienced no tumor growth, suggesting induction of systemic immunity. Moreover, splenocytes prepared from vaccinated C3H mice demonstrated higher proliferative activity and produced elevated levels of TH1 cytokines compared with those from control mice. An in vitro subset analysis of splenocytes from vaccinated mice demonstrated a significant increase in the CD3(+)CD8(+) but not the CD3(+)CD4(+) cell population (P=0.019). Thus Ad-mda7 treatment of syngeneic tumors induces tumor cell death and promotes immune activation, leading to anticancer immunity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0929-1903
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
753-61
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16543916-Adenoviridae, pubmed-meshheading:16543916-Animals, pubmed-meshheading:16543916-Apoptosis, pubmed-meshheading:16543916-Cancer Vaccines, pubmed-meshheading:16543916-Cell Line, pubmed-meshheading:16543916-Cell Line, Tumor, pubmed-meshheading:16543916-Cell Proliferation, pubmed-meshheading:16543916-Cytokines, pubmed-meshheading:16543916-Female, pubmed-meshheading:16543916-Fibrosarcoma, pubmed-meshheading:16543916-Gene Therapy, pubmed-meshheading:16543916-Genetic Vectors, pubmed-meshheading:16543916-Immunocompetence, pubmed-meshheading:16543916-Injections, Intralesional, pubmed-meshheading:16543916-Interleukins, pubmed-meshheading:16543916-Mice, pubmed-meshheading:16543916-Mice, Inbred C3H, pubmed-meshheading:16543916-Spleen, pubmed-meshheading:16543916-Th1 Cells, pubmed-meshheading:16543916-Transplantation, Isogeneic, pubmed-meshheading:16543916-Xenograft Model Antitumor Assays
pubmed:year
2006
pubmed:articleTitle
Melanoma differentiation-associated gene-7 (mda-7)/interleukin (IL)-24 induces anticancer immunity in a syngeneic murine model.
pubmed:affiliation
Department of Thoracic and Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural