Source:http://linkedlifedata.com/resource/pubmed/id/16538680
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2006-3-22
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| pubmed:abstractText |
Interest in chordate evolution has emphasized a need for a better understanding of the comparative neuroanatomy of invertebrate deuterostomes. However, molecular and genetic approaches to neurobiological studies in these groups are hampered by a lack of neuron-specific molecular markers. A monoclonal antibody, 1E11, is neuron specific and is useful in identification of neural structures in larvae and adults of echinoderms, hemichordates, and urochordates. To identify a neuron-specific gene product, we have characterized the antigen recognized by 1E11. In immunoblots and immunoprecipitations of neural tissue from adult Strongylocentrotus purpuratus, 1E11 recognizes a 57-kDa band. Tandem mass spectrometry of trypsin digests of the 57-kDa band permitted peptide mass mapping and sequencing of five peptides. All of the sequenced peptides, and 12 additional mass-mapped peptides, are found within the open reading frame of a cDNA encoding synaptotagmin B (Sp-SynB). In situ RNA hybridizations with synaptotagmin B probes with S. purpuratus larvae reveal a pattern of expression that is similar to that revealed by the antibody 1E11. Antibodies produced against a bacterially expressed Sp-SynB protein recognize a 57-kDa protein and colocalize with 1E11. When a full-length Sp-SynB cDNA is expressed in chicken embryonic cells, the cells become immunoreactive to 1E11. We conclude that synaptotagmin B is a gene expressed in neurons that has conserved epitopes in other invertebrate deuterostomes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmins
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0021-9967
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| pubmed:author | |
| pubmed:copyrightInfo |
J. Comp. Neurol. 496:244-251, 2006. (c) 2006 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
10
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| pubmed:volume |
496
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
244-51
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16538680-Amino Acid Sequence,
pubmed-meshheading:16538680-Animals,
pubmed-meshheading:16538680-Antibodies,
pubmed-meshheading:16538680-Blotting, Western,
pubmed-meshheading:16538680-Chick Embryo,
pubmed-meshheading:16538680-Drosophila,
pubmed-meshheading:16538680-Gene Expression,
pubmed-meshheading:16538680-Gene Expression Regulation, Developmental,
pubmed-meshheading:16538680-Green Fluorescent Proteins,
pubmed-meshheading:16538680-Immunohistochemistry,
pubmed-meshheading:16538680-Immunoprecipitation,
pubmed-meshheading:16538680-In Situ Hybridization,
pubmed-meshheading:16538680-Larva,
pubmed-meshheading:16538680-Mass Spectrometry,
pubmed-meshheading:16538680-Molecular Weight,
pubmed-meshheading:16538680-Nerve Tissue Proteins,
pubmed-meshheading:16538680-Neurons,
pubmed-meshheading:16538680-Peptide Mapping,
pubmed-meshheading:16538680-Phylogeny,
pubmed-meshheading:16538680-RNA, Messenger,
pubmed-meshheading:16538680-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16538680-Sea Urchins,
pubmed-meshheading:16538680-Synaptotagmins
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| pubmed:year |
2006
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| pubmed:articleTitle |
Neuron-specific expression of a synaptotagmin gene in the sea urchin Strongylocentrotus purpuratus.
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| pubmed:affiliation |
Department of Biology and Biochemistry, University of Victoria, Victoria, British Columbia V8W 3N5, Canada. rburke@uvic.ca
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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