Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-3-15
pubmed:abstractText
Reactivation of the polyomavirus BK (BKV) causes polyomavirus nephropathy (PVN) in kidney transplant (KTx) recipients and may lead to loss of the renal allograft. We have identified two HLA-A*0201-restricted nine-amino-acid cytotoxic T lymphocyte (CTL) epitopes of the BKV major capsid protein VP1, VP1(p44), and VP1(p108). Using tetramer staining assays, we showed that these epitopes were recognized by CTLs in 8 of 10 (VP1(p44)) and 5 of 10 (VP1(p108)) HLA-A*0201+ healthy individuals, while both epitopes elicited a CTL response in 10 of 10 KTx recipients with biopsy-proven PVN, although at variable levels. After in vitro stimulation with the respective peptides, CTLs directed against VP1(p44) were more abundant than against VP1(p108) in most healthy individuals, while the converse was true in KTx recipients with PVN, suggesting a shift in epitope immunodominance in the setting of active BKV infection. A strong CTL response in KTx recipients with PVN appeared to be associated with decreased BK viral load in blood and urine and low anti-BKV antibody titers, while a low or undetectable CTL response correlated with viral persistence and high anti-BKV antibody titers. These results suggest that this cellular immune response is present in most BKV-seropositive healthy individuals and plays an important role in the containment of BKV in KTx recipients with PVN. Interestingly, the BKV CTL epitopes bear striking homology with the recently described CTL epitopes of the other human polyomavirus JC (JCV), JCV VP1(p36) and VP1(p100). A high degree of epitope cross-recognition was present between BKV and corresponding JCV-specific CTLs, which indicates that the same population of cells is functionally effective against these two closely related viruses.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-10452511, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-10769060, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-11238876, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-11517410, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-11535537, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-11751998, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12099383, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12176577, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12181403, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12491153, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12748354, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-12858417, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-1316734, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-14504320, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-14581528, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15115835, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15194802, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15215217, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15264457, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15302959, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15331755, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15367226, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15707414, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15912088, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15947078, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15947079, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15947080, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-15947082, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-16103168, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-16284455, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-6304766, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-6304775, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-7561340, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-8254189, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-8810254, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-9159311, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-9921805, http://linkedlifedata.com/resource/pubmed/commentcorrection/16537617-9932940
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3495-505
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:16537617-Adult, pubmed-meshheading:16537617-Aged, pubmed-meshheading:16537617-Antibody Formation, pubmed-meshheading:16537617-BK Virus, pubmed-meshheading:16537617-Capsid Proteins, pubmed-meshheading:16537617-Epitopes, pubmed-meshheading:16537617-HLA-A Antigens, pubmed-meshheading:16537617-Humans, pubmed-meshheading:16537617-Immunity, Cellular, pubmed-meshheading:16537617-In Situ Hybridization, pubmed-meshheading:16537617-Kidney Diseases, pubmed-meshheading:16537617-Kidney Transplantation, pubmed-meshheading:16537617-Kinetics, pubmed-meshheading:16537617-Middle Aged, pubmed-meshheading:16537617-Polyomavirus, pubmed-meshheading:16537617-Polyomavirus Infections, pubmed-meshheading:16537617-T-Lymphocytes, Cytotoxic, pubmed-meshheading:16537617-Transplantation, Homologous, pubmed-meshheading:16537617-Viral Load
pubmed:year
2006
pubmed:articleTitle
Interplay of cellular and humoral immune responses against BK virus in kidney transplant recipients with polyomavirus nephropathy.
pubmed:affiliation
Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural