Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-3-14
pubmed:abstractText
Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity, reaching 50% inhibition with 46.7 +/- 8.3 microM SIN-1 for 8.7 microM S1, that is, at a SIN-1/S1 molar ratio of approximately 5.5. The inhibition was due to the peroxynitrite produced by SIN-1 decomposition because (1) decomposed SIN-1 was found to have no effect on S1 ATPase activity, (2) addition of SIN-1 in the presence of superoxide dismutase and catalase fully prevented inhibition by SIN-1, and (3) micromolar pulses of chemically synthesized peroxynitrite produced inhibition of F-actin-stimulated S1 Mg(2+)-ATPase activity. In parallel, SIN-1 produced the inhibition of the nonphysiological Ca(2+)-dependent and K(+)/EDTA-dependent S1 ATPase activity of S1 and, therefore, suggested that the inhibition of F-actin-stimulated S1 Mg(2+)-ATPase activity is produced by the oxidation of highly reactive cysteines of S1 (Cys(707) and Cys(697)), located close to the catalytic center. This point was further confirmed by the titration of S1 cysteines with 5,5'-dithiobis(2-nitrobenzoic acid) and by the parallel decrease of Cys(707) labeling by 5-(iodoacetamido)fluorescein, and it was reinforced by the fact that other common protein modifications produced by peroxynitrite, for example, protein carbonyl and nitrotyrosine formation, were barely detected at the concentrations of SIN-1 that produced more than 50% inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity. Differential scanning calorimetry of S1 (untreated and treated with different SIN-1 concentrations) pointed out that SIN-1, at concentrations that generate micromolar peroxynitrite fluxes, impaired the ability of ADP.V(1) to induce the intermediate catalytic transition state and also produced the partial unfolding of S1 that leads to an enhanced susceptibility of S1 to trypsin digestion, which can be fully protected by 2 mM GSH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/3-morpholino-sydnonimine, http://linkedlifedata.com/resource/pubmed/chemical/3-nitrotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate, http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Cysteine, http://linkedlifedata.com/resource/pubmed/chemical/Magnesium, http://linkedlifedata.com/resource/pubmed/chemical/Molsidomine, http://linkedlifedata.com/resource/pubmed/chemical/Myosin Subfragments, http://linkedlifedata.com/resource/pubmed/chemical/Peroxynitrous Acid, http://linkedlifedata.com/resource/pubmed/chemical/Skeletal Muscle Myosins, http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3794-804
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16533063-Actins, pubmed-meshheading:16533063-Adenosine Diphosphate, pubmed-meshheading:16533063-Adenosine Triphosphatases, pubmed-meshheading:16533063-Animals, pubmed-meshheading:16533063-Catalysis, pubmed-meshheading:16533063-Cysteine, pubmed-meshheading:16533063-Dose-Response Relationship, Drug, pubmed-meshheading:16533063-Enzyme Stability, pubmed-meshheading:16533063-Magnesium, pubmed-meshheading:16533063-Molsidomine, pubmed-meshheading:16533063-Myosin Subfragments, pubmed-meshheading:16533063-Oxidation-Reduction, pubmed-meshheading:16533063-Peroxynitrous Acid, pubmed-meshheading:16533063-Protein Folding, pubmed-meshheading:16533063-Rabbits, pubmed-meshheading:16533063-Skeletal Muscle Myosins, pubmed-meshheading:16533063-Temperature, pubmed-meshheading:16533063-Time Factors, pubmed-meshheading:16533063-Tyrosine
pubmed:year
2006
pubmed:articleTitle
Inhibition of skeletal muscle S1-myosin ATPase by peroxynitrite.
pubmed:affiliation
Centre for Marine Sciences, Departamento de Química e Bioquímica, FCT, Universidade do Algarve 8005-139, Faro, Portugal.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't