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rdf:type |
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lifeskim:mentions |
umls-concept:C0037083,
umls-concept:C0046319,
umls-concept:C0086982,
umls-concept:C0127400,
umls-concept:C0220905,
umls-concept:C0334227,
umls-concept:C0376358,
umls-concept:C0439064,
umls-concept:C1709059,
umls-concept:C1710082,
umls-concept:C1882071
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pubmed:issue |
11
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pubmed:dateCreated |
2006-3-14
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pubmed:abstractText |
2-Methoxyestradiol (2-ME(2)), a promising anticancer drug, induces growth arrest and apoptosis in various androgen-dependent (LNCaP) and -independent (DU145 and PC-3) prostate cancer cell lines. Moreover, flow cytometric analysis indicated a novel dual impact of 2-ME(2) on the cell division cycle of prostate cancer cells. Chronic exposure of high doses of 2-ME(2) enhance the accumulation of cells in S and G2/M phases, while cell numbers in the G1 phase were reduced significantly by this treatment. Because cyclin B1 overexpression, induction of cdc2 phosphorylation, and its regulatory proteins wee1 and phospho-cdc25C (interphase and mitotic forms) by 2-ME(2) treatment correlated with the induction of apoptosis, growth arrest at the G2/M phase, and accumulation of the S phase, we reasoned that cyclin B1 and cdc2 phosphorylation and its upstream regulatory molecular networks may be associated with the ultimate impacts of 2-ME(2). Because phosphorylation of cdc2 and upregulation of wee1 by 2-ME(2) can be abolished by both extracellular receptor kinase (ERK) inhibitor (U0126) and c-Jun N-terminal kinase (JNK) inhibitor (SP600125), our studies indicate that the 2-ME(2)-induced upregulation of wee1 and subsequent cdc2 phosphorylation are mediated through mitogen-activated protein kinase (MAPK)-ERK-JNK signaling pathways.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-methoxyestradiol,
http://linkedlifedata.com/resource/pubmed/chemical/CCNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDC2 Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/CDC25C protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin B,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin B1,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin Modulators,
http://linkedlifedata.com/resource/pubmed/chemical/WEE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/cdc25 Phosphatases
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3703-13
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16533053-Apoptosis,
pubmed-meshheading:16533053-CDC2 Protein Kinase,
pubmed-meshheading:16533053-Cell Cycle,
pubmed-meshheading:16533053-Cell Cycle Proteins,
pubmed-meshheading:16533053-Cell Division,
pubmed-meshheading:16533053-Cyclin B,
pubmed-meshheading:16533053-Cyclin B1,
pubmed-meshheading:16533053-Cytoplasm,
pubmed-meshheading:16533053-Estradiol,
pubmed-meshheading:16533053-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:16533053-G2 Phase,
pubmed-meshheading:16533053-Humans,
pubmed-meshheading:16533053-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:16533053-Male,
pubmed-meshheading:16533053-Models, Biological,
pubmed-meshheading:16533053-Nuclear Proteins,
pubmed-meshheading:16533053-Phosphorylation,
pubmed-meshheading:16533053-Prostatic Neoplasms,
pubmed-meshheading:16533053-Protein-Tyrosine Kinases,
pubmed-meshheading:16533053-S Phase,
pubmed-meshheading:16533053-Signal Transduction,
pubmed-meshheading:16533053-Tubulin Modulators,
pubmed-meshheading:16533053-Tumor Cells, Cultured,
pubmed-meshheading:16533053-Up-Regulation,
pubmed-meshheading:16533053-cdc25 Phosphatases
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pubmed:year |
2006
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pubmed:articleTitle |
Modulation of cell-cycle regulatory signaling network by 2-methoxyestradiol in prostate cancer cells is mediated through multiple signal transduction pathways.
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pubmed:affiliation |
Cancer Research Unit, Veterans Affairs Medical Center, Kansas City, Missouri, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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